Gleason M K, Metzenberg R L
Genetics. 1974 Oct;78(2):645-59. doi: 10.1093/genetics/78.2.645.
Mutants of Neurospora crassa have been isolated that lack the repressible alkaline phosphatase, but, unlike nuc-1 and nuc-2 mutants, are able to make the repressible acid phosphatase and the repressible phosphate permease under conditions of derepression (phosphate deprivation). The new mutants, called pho-2, map in Linkage Group V, and are unlinked to the putative control mutants, nuc-1, nuc-2-pcon(c), and preg(c). Three of the pho-2 mutants do not make detectable amounts of repressible alkaline phosphatase, but the fourth makes about 1% of the level found in wild type. The small amount of alkaline phosphatase made by this strain appears to be qualitatively similar or identical to the wild-type enzyme, as judged by electrophoretic mobility, heat stability, and titration with specific antibody to the wild-type enzyme. Several revertants of this strain have been examined in the same way, and the alkaline phosphatase of these strains also appears to be qualitatively normal. Reversion events can occur at, or near, the pho-2 locus, but also occur in at least two unlinked sites (suppressor mutations). One suppressor maps very close to nuc-1.
已分离出粗糙脉孢菌的突变体，这些突变体缺乏可阻遏的碱性磷酸酶，但与nuc - 1和nuc - 2突变体不同的是，在去阻遏条件下（磷酸盐缺乏）能够产生可阻遏的酸性磷酸酶和可阻遏的磷酸盐通透酶。这些新的突变体称为pho - 2，定位于连锁群V，与假定的控制突变体nuc - 1、nuc - 2 - pcon(c)和preg(c)不连锁。其中三个pho - 2突变体无法产生可检测量的可阻遏碱性磷酸酶，但第四个突变体产生的量约为野生型的1%。通过电泳迁移率、热稳定性以及用野生型酶的特异性抗体滴定判断，该菌株产生的少量碱性磷酸酶在性质上似乎与野生型酶相似或相同。已以同样的方式检测了该菌株的几个回复突变体，这些菌株的碱性磷酸酶在性质上似乎也正常。回复事件可发生在pho - 2基因座处或其附近，但也发生在至少两个不连锁的位点（抑制突变）。其中一个抑制基因定位于非常靠近nuc - 1的位置。
