L-丙氨酸脱氢酶催化的L-丙氨酸氟衍生物生物转化机理研究中的同位素效应
Isotopic effects in mechanistic studies of biotransformations of fluorine derivatives of L-alanine catalysed by L-alanine dehydrogenase.
作者信息
Szymańska-Majchrzak Jolanta, Pałka Katarzyna, Kańska Marianna
机构信息
Department of Biochemistry, 2nd Faculty of Medicine, Medical University of Warsaw, 101 Żwirki i Wigury Ave., 02-089 Warsaw, Poland.
Department of Chemistry, University of Warsaw, 1 Pasteur Str., 02-093 Warsaw, Poland.
出版信息
Appl Radiat Isot. 2017 May;123:21-25. doi: 10.1016/j.apradiso.2017.02.003. Epub 2017 Feb 12.
Synthesis of 3-fluoro-[2-H]-L-alanine (3-F-[H]-L-Ala) in reductive amination of 3-fluoropyruvic acid catalysed by L-alanine dehydrogenase (AlaDH) was described. Fluorine derivative was used to study oxidative deamination catalysed by AlaDH applied kinetic (for 3-F-L-Ala in HO - KIE's on V: 1.1; on V/K: 1.2; for 3-F-L-Ala in HO - on V: 1.4; on V/K: 2.1) and solvent isotope effect methods (for 3-F-L-Ala - SIE's on V: 1.0; on V/K: 0.87; for 3-F-[2-H]-L-Ala - on V: 1.4; on V/K: 1.5). Studies explain some details of reaction mechanism.
描述了在L-丙氨酸脱氢酶（AlaDH）催化下3-氟丙酮酸还原胺化合成3-氟-[2-H]-L-丙氨酸（3-F-[H]-L-Ala）的过程。氟衍生物用于研究AlaDH催化的氧化脱氨反应，采用了动力学方法（对于HO中的3-F-L-Ala，V的动力学同位素效应为1.1；V/K的动力学同位素效应为1.2；对于HO中的3-F-L-Ala，V的动力学同位素效应为1.4；V/K的动力学同位素效应为2.1）和溶剂同位素效应方法（对于3-F-L-Ala，V的溶剂同位素效应为1.0；V/K的溶剂同位素效应为0.87；对于3-F-[2-H]-L-Ala，V的溶剂同位素效应为1.4；V/K的溶剂同位素效应为1.5）。这些研究解释了反应机理的一些细节。
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