Coexistence of and Truncated on Different Plasmids in a Isolate in China.

To describe the genetic environment, transferability, and antibiotic susceptibility of one clinical isolate harboring both and on different plasmids from a Chinese hospital. The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using Etest and PCR. The plasmids harboring and were analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis, and hybridization with specific probes. Plasmid DNA was sequenced using Pacbio RS II and annotated using RAST. RJ119, carrying both and , was resistant to almost all carbapenems, cephalosporins, fluoroquinolone, and aminoglycosides and belonged to ST307. was located on a 61,748-bp IncL/M conjugative plasmid, which displayed overall nucleotide identity (99%) to pKPN-E1-Nr.7. was located on a 335,317-bp conjugative plasmid, which was a fusion of a -harboring InA/C plasmid pNDM-US (140,825 bp, 99% identity) and an IncFIB plasmid pKPN-c22 (178,563 bp, 99% identity). The transconjugant RJ119-1 harboring was susceptible to carbapenem, and there was an insertion of IS into the gene. This is the first report of the coexistence of and in one clinical isolate in China. OXA-48 in RJ119 contributed to the majority to its high resistance to carbapenems, whereas NDM-1 remained unexpressed, most likely due to the insertion of IS. Our results provide new insight for the relationship between genetic diagnosis and clinical treatment. They also indicate that increased surveillance of is urgently needed in China.