Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, China.
Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
J Glob Antimicrob Resist. 2018 Jun;13:24-27. doi: 10.1016/j.jgar.2017.10.021. Epub 2017 Nov 2.
This study characterised a bla-producing Escherichia coli isolate from a companion dog.
The E. coli strain was isolated from a surveillance study of carbapenem-resistant Gram-negative bacteria from companion animals in the Animal Teaching Hospital of China Agricultural University (Beijing, China) in 2013. Species identification was performed using an API 20E system and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by agar dilution. Multilocus sequence typing (MLST) was performed and various carbapenemase genes, including bla, bla, bla, bla, bla, bla, bla, bla, bla, bla, bla, bla, bla, bla, bla, bla and bla were screened by PCR. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting as well as a modified random primer walking strategy were performed to analyse the location and genetic environment of bla.
An E. coli isolate belonging to ST167 was found to be positive for the bla gene and exhibited resistance to β-lactams, tetracycline, gentamicin, fosfomycin and ciprofloxacin. The bla gene in this strain was located on an ca. 150-kb plasmid and the flanking sequences of the bla-carrying region (a common gene cluster, ΔISAba125-bla-ble-trpF-ΔdsbC) exhibited >99% identity to the corresponding regions of bla-harboring plasmids in nosocomial E. coli ST131 isolates.
This is the first report of bla-producing ST167 E. coli in a companion dog. Companion animals harbouring carbapenemase-producing isolates are an upcoming public health threat and it is worthy paying attention to the emergence of carbapenem resistance in companion animals.
本研究对从伴侣犬中分离出的产 bla 的大肠杆菌进行了特征描述。
2013 年，从中国农业大学动物教学医院（北京）的动物中耐碳青霉烯类的革兰氏阴性菌监测研究中分离出大肠杆菌菌株。采用 API 20E 系统和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF/MS）进行物种鉴定。通过琼脂稀释法测定各种抗菌药物的最小抑菌浓度（MIC）。进行多位点序列分型（MLST），并通过 PCR 筛选各种碳青霉烯酶基因，包括 bla、bla、bla、bla、bla、bla、bla、bla、bla、bla、bla、bla、bla、bla、bla、bla 和 bla。通过 S1 核酸酶脉冲场凝胶电泳（S1-PFGE）和 Southern 印迹以及改良的随机引物步行策略分析 bla 的位置和遗传环境。
发现属于 ST167 的大肠杆菌分离株 bla 基因阳性，对β-内酰胺类、四环素、庆大霉素、磷霉素和环丙沙星耐药。该菌株的 bla 基因位于一个约 150kb 的质粒上，携带 bla 的区域侧翼序列（一个常见的基因簇，ΔISAba125-bla-ble-trpF-ΔdsbC）与医院获得性大肠杆菌 ST131 分离株中 bla 携带质粒的相应区域具有 >99%的同一性。
这是首例在伴侣犬中发现 bla 产 ST167 大肠杆菌的报告。携带产碳青霉烯酶分离株的伴侣动物是一个即将出现的公共卫生威胁，值得关注伴侣动物中碳青霉烯类耐药性的出现。
