Zhang Zhanquan, Chen Jian, Li Boqiang, He Chang, Chen Yong, Tian Shiping
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences Beijing, China.
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of SciencesBeijing, China; College of Life Sciences, University of Chinese Academy of SciencesBeijing, China.
Front Microbiol. 2017 Feb 2;8:151. doi: 10.3389/fmicb.2017.00151. eCollection 2017.
The limitations of chemical fungicides for the control of postharvest diseases have recently become more apparent. The utilization of antagonistic microorganisms is a promising alternative to that of fungicides to control postharvest decay. In previous studies, the antagonistic yeast has shown excellent effects of biocontrol and great potential for practical application. Adverse conditions, such as oxidative stress, limit the practical application of antagonistic yeast. In this study, we investigated the oxidative stress tolerance of and the associated mechanisms. The results indicated that exogenous oxidative stress has a significant effect on the viability and biocontrol efficiency of . HO-induced oxidative stress led to the accumulation of reactive oxygen species. The results of flow cytometric analysis suggested that apoptosis is responsible for the reduced survival rate of under oxidative stress. Using tests of antioxidant activity, we found that could employ enzymatic systems to resist exogenous oxidative stress. The addition of exogenous glutathione, a non-enzymatic antioxidant, to the media can significantly enhance oxidative tolerance and biocontrol efficiency of .
化学杀菌剂在控制采后病害方面的局限性近来愈发明显。利用拮抗微生物是替代杀菌剂控制采后腐烂的一种有前景的方法。在先前的研究中，拮抗酵母已展现出优异的生物防治效果及巨大的实际应用潜力。诸如氧化应激等不利条件限制了拮抗酵母的实际应用。在本研究中，我们探究了[具体酵母名称未给出]的氧化应激耐受性及相关机制。结果表明，外源氧化应激对[具体酵母名称未给出]的活力和生物防治效率有显著影响。过氧化氢诱导的氧化应激导致活性氧的积累。流式细胞术分析结果表明，细胞凋亡是[具体酵母名称未给出]在氧化应激下存活率降低的原因。通过抗氧化活性测试，我们发现[具体酵母名称未给出]可利用酶系统抵抗外源氧化应激。向培养基中添加外源谷胱甘肽（一种非酶抗氧化剂）可显著提高[具体酵母名称未给出]的氧化耐受性和生物防治效率。
