Samiei Mohammad, Ghasemi Negin, Aghazadeh Marzieh, Divband Baharak, Akbarzadeh Farzaneh
Associate Professor, Department of Endodontics, Dental Faculty, Tabriz University (Medical Sciences), Tabriz, Iran.
Assistant Professor, Department of Endodontics, Dental and Periodontal Research Center, Dental Faculty, Tabriz University (Medical Sciences), Tabriz, Iran.
J Clin Exp Dent. 2017 Feb 1;9(2):e182-e185. doi: 10.4317/jced.53126. eCollection 2017 Feb.
The New compositions of white mineral trioxide aggregate (WMTA) or use of various additives like nanoparticles might affect MTA's ideal characteristics This study was performed to evaluate the cytotoxicity of WMTA and WMTA with Titanium dioxide (TiO2) nanoparticles (1% weight ratio) at different storage times after mixing on human gingival fibroblasts (HGFs).
HGFs were obtained from the attached gingiva of human premolars. HGFs were cultured in Dulbecco's Modified Eagle medium, supplemented with 10% fetal calf serum, penicillin and streptomycin. The cells were exposed to WMTA (groups 1 and 2) and WMTA+TiO2 (groups 3 and 4). The fifth and sixth groups served as controls. Each group contained 15 wells. After 24h (groups 1, 3 and 5) and 48 h (groups 2, 4 and 6) of exposure, HGF viability was determined by Mosmann's tetrazolium toxicity (MTT) assay. Statistical analysis of the data was performed by using one-way analysis of variance and Tukey post hoc test, with significance of < 0.05.
With both materials, the viability of HGFs significantly decrased with increasing the incubation time from 24h to 48 h (<0.05). There was no significant difference between the materials regarding HGF viability (>0.05).
Under the limitations of the present study, incorporation of TiO2 nanoparticles into MTA at 1 wt% had no negative effect on its biocompatibility. Cytotoxicity, fibroblast, MTA, MTT assay, nanoparticle, TiO2.
白色三氧化矿物凝聚体（WMTA）的新成分或各种添加剂（如纳米颗粒）的使用可能会影响MTA的理想特性。本研究旨在评估WMTA以及与二氧化钛（TiO2）纳米颗粒（重量比1%）混合后的WMTA在不同储存时间对人牙龈成纤维细胞（HGFs）的细胞毒性。
HGFs取自人前磨牙的附着龈。HGFs在补充有10%胎牛血清、青霉素和链霉素的杜尔贝科改良伊格尔培养基中培养。细胞分别暴露于WMTA（第1组和第2组）和WMTA+TiO2（第3组和第4组）。第5组和第6组作为对照。每组包含15个孔。暴露24小时（第1、3和5组）和48小时（第2、4和6组）后，通过莫斯曼四唑盐毒性（MTT）试验测定HGF的活力。数据采用单因素方差分析和Tukey事后检验进行统计分析，显著性水平为<0.05。
两种材料处理后，随着孵育时间从24小时增加到48小时，HGF的活力均显著降低（<0.05）。在HGF活力方面，两种材料之间无显著差异（>0.05）。
在本研究的局限性下，以1 wt%的比例将TiO2纳米颗粒掺入MTA对其生物相容性没有负面影响。细胞毒性、成纤维细胞、MTA、MTT试验、纳米颗粒、TiO2 。
