Samiei Mohammad, Shahi Shahriar, Ghasemi Negin, Dastmalchi Siavoush, Bargahi Nasrin, Asgary Saeed
Department of Endodontics, Dental School, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Endodontics, Dental and Periodontal Research Center, Dental School, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran Endod J. 2018 Winter;13(1):37-41. doi: 10.22037/iej.v13i1.16913.
: The aim of the present study was to evaluate the genotoxicity of mineral trioxide aggregate (MTA) after adding different concentrations of disodium hydrogen phosphate and silver nanoparticles using the Ames test.
: TA100 strain of was used to evaluate mutagenicity of experimental materials with and without S9 mix fraction. The materials tested in this study consisted of MTA, MTA/disodium hydrogen phosphate and MTA/silver nanoparticles at 0.1, 0.01, 0.001 and 0.0001 concentrations. Negative and positive control groups consisted of 1% dimethyl sulfoxide and sodium azide with 2-aminoanthracene, respectively. The number of colonies per plate was determined. If the ratio of the number of histidine-revertant colonies to spontaneous revertants of the negative control colonies was ≥2, the material was regarded a mutagenic agent.
: In all the concentrations of the three tested materials, the Ames test failed to detect mutations.
: Under the limitations of the present study, MTA/disodium hydrogen phosphate and MTA/silver nanoparticles were biocompatible in relation to mutagenicity.
本研究的目的是通过艾姆斯试验评估添加不同浓度磷酸氢二钠和银纳米颗粒后三氧化矿物凝聚体（MTA）的遗传毒性。
使用TA100菌株评估有无S9混合组分时实验材料的致突变性。本研究中测试的材料包括浓度为0.1、0.01、0.001和0.0001的MTA、MTA/磷酸氢二钠和MTA/银纳米颗粒。阴性和阳性对照组分别由1%二甲基亚砜和叠氮化钠与2-氨基蒽组成。测定每平板的菌落数。如果组氨酸回复突变菌落数与阴性对照菌落自发回复突变数的比值≥2，则该材料被视为诱变剂。
在三种测试材料的所有浓度下，艾姆斯试验均未检测到突变。
在本研究的局限性下，MTA/磷酸氢二钠和MTA/银纳米颗粒在致突变性方面具有生物相容性。
