普洛罗特MTA、MTA根尖封闭剂和波特兰水泥对人牙龈成纤维细胞的细胞毒性评估
Cytotoxicity Evaluation of Proroot MTA, Root MTA and Portland Cement on Human Gingival Fibroblasts.
作者信息
Sharifian Mohammad Reza, Ghobadi Mehrnoosh, Shokouhinejad Noushin, Assadian Hadi
机构信息
Department of Endodontics, School of Dentistry, Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Iran Endod J. 2007 Fall;2(3):91-4. Epub 2007 Oct 2.
INTRODUCTION
The aim of this study was to compare the cytotoxicity of ProRoot MTA (PMTA), Root MTA (RMTA) and Portland cement (PC) on human gingival fibroblasts (HGFs).
MATERIALS AND METHODS
The extracts of the test materials were placed close to human gingival fibroblasts (HGFs) within 96-well plates. Cellular viability was assessed using MTT assay in different intervals (freshly mixed, 4, 24, and 168 hours after mixing). The data were analyzed using the One-way ANOVA and Tukey's test at 95% significance level.
RESULTS
It was indicated that there was not a significant difference in cytotoxicity of test materials (p>0.05). In addition, there was not a statistically significant difference between different time intervals within each group (p>0.05).
CONCLUSION
PMTA, RMTA and PC showed comparative biocompatibility while evaluated in-vitro.
引言
本研究旨在比较ProRoot MTA（PMTA）、Root MTA（RMTA）和波特兰水泥（PC）对人牙龈成纤维细胞（HGFs）的细胞毒性。
材料与方法
将测试材料的提取物置于96孔板中靠近人牙龈成纤维细胞（HGFs）的位置。在不同时间间隔（新鲜混合时、混合后4小时、24小时和168小时）使用MTT法评估细胞活力。数据采用单因素方差分析和Tukey检验进行分析，显著性水平为95%。
结果
结果表明，测试材料的细胞毒性无显著差异（p>0.05）。此外，每组不同时间间隔之间也无统计学显著差异（p>0.05）。
结论
在体外评估时，PMTA、RMTA和PC表现出相当的生物相容性。
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