Taheran Leila, Zarrini Gholamreza, Khorram Sirous, Zakerhamidi Mohammad Sadegh
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Research Institute for Applied Physics and Astronomy, University of Tabriz, Tabriz, Iran.
Iran J Microbiol. 2016 Aug;8(4):257-262.
Biomaterials are widely used in medical devices such as urinary catheters. One of the main problems associated with long term using of the urinary catheters is biofilm formation on their surfaces. Many techniques have been presented to reduce the biofilm formation. One of the most revolutionary techniques allowing such surface fictionalization is plasma surface modification.
In this study, a glow discharge plasma (GDP) effect on biofilm formation on the surface of urinary catheter in the pressure of 1.6 × 10 Torr of nitrogen, discharge voltage about 1.2 kV and current of 150 mA for 20 minutes has been investigated. Crystal violet binding assay and sonication method were performed in order to evaluate the amount of biofilm formation on tested biomaterials.
Characterization of modified surfaces by Attenuated Total Reflectance Fourier Transform Infrared Spectrometry (ATR-FTIR) and atomic force microscopy (AFM) revealed a noticeable change in hydrophobicity and roughness of catheter surfaces achieved by nitrogen plasma. The results of crystal violet binding assay and sonication method showed that the amount of biofilm formation on modified surface was about 86% less than the pristine sample.
Plasma surface modification can reduce the risk of infections in patients with long-term use of urinary catheters.
生物材料广泛应用于诸如导尿管等医疗器械中。长期使用导尿管所带来的主要问题之一是其表面形成生物膜。人们已经提出了许多技术来减少生物膜的形成。其中最具革命性的能够实现这种表面功能化的技术之一是等离子体表面改性。
在本研究中，研究了在1.6×10托的氮气压力、约1.2 kV的放电电压和150 mA的电流下，辉光放电等离子体（GDP）对导尿管表面生物膜形成的影响，处理时间为20分钟。为了评估受试生物材料上生物膜的形成量，进行了结晶紫结合试验和超声处理法。
通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）和原子力显微镜（AFM）对改性表面进行表征，结果显示通过氮等离子体处理，导尿管表面的疏水性和粗糙度发生了显著变化。结晶紫结合试验和超声处理法的结果表明，改性表面上生物膜的形成量比原始样品减少了约86%。
等离子体表面改性可以降低长期使用导尿管患者的感染风险。
