Zhang He-Cai, Liu Tong-Yi, Shi Chang-Ying, Chen Guang-Wen, Liu De-Zeng
College of Life Sciences, Henan Normal University, No. 46, Jianshe East Road, Xinxiang, 453007, China.
Institute of Natural Resources, Heilongjiang Academy of Science, Harbin, 150031, China.
Bull Environ Contam Toxicol. 2017 Apr;98(4):484-488. doi: 10.1007/s00128-016-2027-9. Epub 2017 Feb 16.
The aim of this study was to evaluate the genotoxic potential of an urban river - the Wei River in Xinxiang, China using randomly amplified polymorphic DNA (RAPD) assay in planarians. The results showed that the total number of polymorphic bands and varied bands in RAPD patterns of treated planarians decreased with the water sample site far away from the sewage outlet of a factory. In addition, the genome template stability of treated groups decreased and the degree of the decline was negatively related to the distance between the sample site and the sewage outlet, suggesting that the Wei River water had genotoxicity effects on planarians and strengthening the management of the Wei River was necessary. Furthermore, this work also indicated that RAPD assay in planarians was a very promising test for environmental monitoring studies.
本研究的目的是利用涡虫的随机扩增多态性DNA（RAPD）分析来评估中国新乡一条城市河流——渭河的遗传毒性潜力。结果表明，处理后的涡虫RAPD图谱中多态性条带和可变条带的总数随着水样采集点距工厂污水排放口距离的增加而减少。此外，处理组的基因组模板稳定性降低，且下降程度与采样点和污水排放口之间的距离呈负相关，这表明渭河水对涡虫具有遗传毒性作用，加强渭河管理很有必要。此外，这项工作还表明，涡虫的RAPD分析对于环境监测研究是一种非常有前景的检测方法。
