Kharlamenko Vladimir I, Svetashev Vasily I, Tarasova Tatyana S
A.V. Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, ul. Palchevskogo 17, Vladivostok, 690041, Russia.
Lipids. 2017 Apr;52(4):345-352. doi: 10.1007/s11745-017-4237-2. Epub 2017 Feb 16.
Fatty acids (FA) of agglutinating foraminifera of two genera, Bathysiphon and Rhabdammina, collected from a depth of 3307-3377 m in the Kuril Basin, Sea of Okhotsk, and adjacent deep-sea waters of the Pacific Ocean, were analyzed. In lipids of two Bathysiphon species, in addition to common FA typical for marine organisms, the uncommon ∆4 and ∆7 monoenoic acids and ∆4,7-dienoic acid were found in noticeable amounts. FA of two Rhabdammina species included also such uncommon acids as ∆5,8,11,14-21:4, ∆5,8,11,14-22:4, and ∆5,8,11,14-23:4, which are homologues to arachidonic acid. High levels of cis-vaccenic and arachidonic acids were typical for lipids of all the studied species. At the same time, we observed a substantial difference in FA composition between members of these two genera. Using GC and GC-MS of FAME, pyrrolidide and DMOX derivatives, the structures of 10 new FA were confirmed: ∆4-i-16:1 (1.1-0.6%), ∆7-i-21:1 (0.4%), ∆7-ai-21:1 (0.3-0.8%), ∆4,11-18:2 (0.3-0.9%), ∆4,7-20:2 (8.8%), ∆7,12-20:2 (0.4-0.6%), ∆4,7-21:2 (0.6%), ∆7,12-22:2 (6.4-2.1%); ∆5,8,11,14-22:4 (1.2-2.0%), and ∆5,8,11,14-23:4 (1.7-2.3%). The origin of the new FA and the role of foraminifera FA in deep-sea communities are discussed.
对从鄂霍次克海千岛海盆3307 - 3377米深处以及太平洋邻近深海海域采集的两个属（深海管虫属和杆杖虫属）的凝集有孔虫的脂肪酸（FA）进行了分析。在两种深海管虫属物种的脂质中，除了海洋生物常见的典型脂肪酸外，还发现了含量显著的罕见的∆4和∆7单烯酸以及∆4,7 - 二烯酸。两种杆杖虫属物种的脂肪酸还包括诸如∆5,8,11,14 - 21:4、∆5,8,11,14 - 22:4和∆5,8,11,14 - 23:4等罕见酸，它们是花生四烯酸的同系物。所有研究物种的脂质中，顺式- 11 - 十八碳烯酸和花生四烯酸含量都很高。同时，我们观察到这两个属的成员之间脂肪酸组成存在显著差异。利用脂肪酸甲酯（FAME）、吡咯烷化物和DMOX衍生物的气相色谱（GC）和气相色谱 - 质谱联用（GC - MS）技术，确认了10种新脂肪酸的结构：∆4 - i - 16:1（1.1 - 0.6%）、∆7 - i - 21:1（0.4%）、∆7 - ai - 21:1（0.3 - 0.8%）、∆4,11 - 18:2（0.3 - 0.9%）、∆4,7 - 20:2（8.8%）、∆7,12 - 20:2（0.4 - 0.6%）、∆4,7 - 21:2（0.6%）、∆7,12 - 22:2（6.4 - 2.1%）；∆5,8,11,14 - 22:4（1.2 - 2.0%）和∆5,8,11,14 - 23:4（1.7 - 2.3%）。讨论了新脂肪酸的来源以及有孔虫脂肪酸在深海群落中的作用。
