Energy Transformed National Research Flagship, CSIRO Marine and Atmospheric Research, GPO Box 1538, Hobart TAS 7001, Australia.
Phytochemistry. 2011 Aug;72(11-12):1460-5. doi: 10.1016/j.phytochem.2011.04.001. Epub 2011 May 3.
A series of unusual odd-chain fatty acids (OC-FA) were identified in two thraustochytrid strains, TC 01 and TC 04, isolated from waters off the south east coast of Tasmania, Australia. FA compositions were determined by capillary GC and GC-MS, with confirmation of polyunsaturated fatty acids (PUFA) structure performed by analysis of 4,4-dimethyloxazoline derivatives. PUFA constituted 68-74% of the total FA, with the essential PUFA; eicosapentaenoic acid (20:5ω3, EPA), arachidonic acid (20:4ω6, AA) and docosahexaenoic acid (22:6ω3, DHA), accounting for 42-44% of the total FA. High proportions of the saturated OC-FA 15:0 (7.1% in TC 01) and 17:0 (6.2% in TC 04) were detected. The OC-FA 17:1ω8 was present at 2.8% in TC 01. Of particular interest, the C₂₁ PUFA 21:5ω5 and 21:4ω7 were detected at 3.5% and 4.1%, respectively, in TC 04. A proposed biosynthesis pathway for these OC-PUFA is presented. It is possible that the unsaturated OC-PUFA found previously in a number of marine animals were derived from dietary thraustochytrids and they could be useful biomarkers in environmental and food web studies.
从澳大利亚塔斯马尼亚州东南海岸的水域中分离到的两个硫球藻菌株 TC 01 和 TC 04 中鉴定出了一系列不寻常的奇数链脂肪酸 (OC-FA)。通过毛细管 GC 和 GC-MS 确定 FA 组成，并通过分析 4,4-二甲基恶唑啉衍生物对多不饱和脂肪酸 (PUFA) 结构进行确认。PUFA 构成总 FA 的 68-74%，必需 PUFA：二十碳五烯酸 (20:5ω3，EPA)、花生四烯酸 (20:4ω6，AA) 和二十二碳六烯酸 (22:6ω3，DHA)，占总 FA 的 42-44%。检测到高比例的饱和 OC-FA 15:0（TC 01 中为 7.1%）和 17:0（TC 04 中为 6.2%）。OC-FA 17:1ω8 在 TC 01 中含量为 2.8%。特别值得注意的是，在 TC 04 中分别检测到 21:5ω5 和 21:4ω7 这两种 C₂₁ PUFA，含量分别为 3.5%和 4.1%。提出了这些 OC-PUFA 的生物合成途径。以前在许多海洋动物中发现的不饱和 OC-PUFA 可能来自饮食中的硫球藻，它们可能是环境和食物网研究中的有用生物标志物。
