Moura Carlos T M, Batista-Lima Francisco J, Brito Teresinha S, Silva Alfredo A V, Ferreira Luan C, Roque Cássia R, Aragão Karoline S, Havt Alexandre, Fonseca Francisco N, Leal Luzia K A M, Magalhães Pedro J C
Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Department of Pharmacy, School of Pharmacy, Odontology and Nursing, Federal University of Ceará, Fortaleza, Brazil.
J Pharm Pharmacol. 2017 Jun;69(6):722-732. doi: 10.1111/jphp.12689. Epub 2017 Feb 17.
Justicia pectoralis is a plant useful for the treatment of respiratory diseases. Here, we studied the antiasthmatic properties of a standardized extract of J. pectoralis (Jp).
Ovalbumin (OVA)-sensitized rats were actively challenged with saline or OVA to study airway hyper-responsiveness after oral treatment with saline or Jp. The ability of Jp to inhibit hyper-reactivity was evaluated in isolated trachea mounted in isolated organ bath chamber.
Using KCl or carbachol as contractile agents, tracheal rings of OVA-challenged rats contracted with higher magnitude than trachea of rats challenged with saline. Such hyper-responsive phenotype of OVA-challenged tissues decreased with Jp administration. In Ca -free medium, Jp or its major constituent coumarin inhibited preferentially the contractions induced by Ca addition in tissues of OVA-challenged rats stimulated with KCl or acetylcholine. In tissues depleted of their internal Ca stores in the presence of thapsigargin, Jp inhibited the contraction induced by capacitative Ca entry. By gavage, Jp abolished the increase caused by challenge with OVA on the levels of IL-1β and TNF-α in the bronchoalveolar fluid and also impaired the changes in gene expression of canonical transient receptor proteins.
Jp has antiasthmatic properties in an experimental model that reproduces tracheal hyper-reactivity.
爵床是一种可用于治疗呼吸系统疾病的植物。在此，我们研究了爵床标准化提取物（Jp）的抗哮喘特性。
用卵清蛋白（OVA）致敏大鼠，然后用生理盐水或OVA进行主动激发，以研究口服生理盐水或Jp后气道高反应性。在安装于离体器官浴槽中的离体气管中评估Jp抑制高反应性的能力。
以氯化钾或卡巴胆碱作为收缩剂，OVA激发的大鼠气管环收缩幅度高于生理盐水激发的大鼠气管。给予Jp后，OVA激发组织的这种高反应性表型降低。在无钙培养基中，Jp或其主要成分香豆素优先抑制在氯化钾或乙酰胆碱刺激下的OVA激发大鼠组织中因添加钙而诱导的收缩。在毒胡萝卜素存在下使细胞内钙库耗尽的组织中，Jp抑制了由钙库操纵性钙内流诱导的收缩。通过灌胃，Jp消除了OVA激发引起的支气管肺泡灌洗液中白细胞介素-1β和肿瘤坏死因子-α水平的升高，并且还削弱了经典瞬时受体蛋白基因表达的变化。
在再现气管高反应性的实验模型中，Jp具有抗哮喘特性。
