Nunes Tiago Rafael de Sousa, Cordeiro Marina Ferraz, Beserra Fernanda Gomes, de Souza Matheus Landim, da Silva Wliana Alves Viturino, Ferreira Magda Rhayanny Assunção, Soares Luiz Alberto Lira, Costa-Junior Sérgio Dias, Cavalcanti Isabella Macário Ferro, Pitta Maira Galdino da Rocha, Pitta Ivan da Rocha, Rêgo Moacyr Jesus Barreto de Melo
Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation-SuelyGaldino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
Department of Medicine, Federal University of the São Francisco Valley (UNIVASF), Paulo Afonso, Brazil.
Evid Based Complement Alternat Med. 2018 Aug 23;2018:5762368. doi: 10.1155/2018/5762368. eCollection 2018.
Jacq. (Acanthaceae) leaves currently found in the Brazilian north-east are widely used to treat diabetes, menstrual pains, asthma, and other disorders. This work aimed to identify the phytochemical characterization and biological activities of leaf extracts. The plant material was ground and the crude extracts were obtained with water or acetone: water (7:3 v/v), yielding aqueous (JPA), and organic (JPO) extracts. Phytochemical characterization was performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay and trypan blue (TB) exclusion assay in peripheral blood mononuclear cells (PBMCs), BALB/c splenocytes, and neoplastic cells (TOLEDO, K562, DU-145, and PANC-1) at 1, 10, and 100 g/mL. Antibacterial activity was evaluated using the microdilution test to obtain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Cytokines, IFN-, and IL-17A from culture supernatants of BALB/c mice splenocytes were measured by sandwich ELISA. In the TLC analysis, both JPA and JPO extracts presented coumarin and flavonoids. In addition, HPLC was able to identify coumarin, apigenin, and ellagic acid in both extracts. JPO IC was 57.59 ± 1.03 g/mL (MTT) and 69.44 ± 8.08 g/mL (TB) in TOLEDO. MIC value of JPO against and was 500 g/mL. JPO (100 g/mL) significantly inhibited IFN- levels (p=0.03). is a potential candidate to be further investigated as an IFN- inhibitory agent and against and .
目前在巴西东北部发现的爵床科植物叶子被广泛用于治疗糖尿病、痛经、哮喘和其他疾病。这项工作旨在确定叶提取物的植物化学特征和生物活性。将植物材料研磨，用水或丙酮：水（7:3 v/v）获得粗提取物，得到水提取物（JPA）和有机提取物（JPO）。通过薄层色谱法（TLC）和高效液相色谱法（HPLC）进行植物化学特征分析。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法和台盼蓝（TB）排斥试验评估外周血单个核细胞（PBMC）、BALB/c脾细胞和肿瘤细胞（TOLEDO、K562、DU-145和PANC-1）在1、10和100μg/mL浓度下的细胞毒性。使用微量稀释试验评估抗菌活性以获得最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。通过夹心ELISA法测量BALB/c小鼠脾细胞培养上清液中的细胞因子、IFN-和IL-17A。在TLC分析中，JPA和JPO提取物均呈现香豆素和黄酮类化合物。此外，HPLC能够在两种提取物中鉴定出香豆素、芹菜素和鞣花酸。在TOLEDO细胞中，JPO的IC50为57.59±1.03μg/mL（MTT法）和69.44±8.08μg/mL（TB法）。JPO对金黄色葡萄球菌和大肠杆菌的MIC值为500μg/mL。JPO（100μg/mL）显著抑制IFN-水平（p=0.03）。作为一种IFN-抑制剂以及针对金黄色葡萄球菌和大肠杆菌，该植物有进一步研究的潜力。
