Xue Liying, Nan Jinglong, Dong Li, Zhang Cuiying, Li Hui, Na Rentuya, He Huijie, Wang Yadi
Southern Medical University, Guangzhou, Guangdong, China.
Department of Medical Oncology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, China.
Cancer Biomark. 2017;19(2):193-197. doi: 10.3233/CBM-160506.
Esophageal squamous cell carcinoma (ESCC) is one of the most common cause of cancer-associated mortality. Uncovering novel molecular biomarkers that can predict ESCC development will improve personalized therapy.
The goal of the current study was to investigate the expression pattern of miR-483-5p and determine its prognostic value in ESCC.
We first analyzed miRNA-seq data obtained from the Cancer Genome Atlas (TCGA) cohort to evaluate the prognostic value of miR-483-5p in ESCC. Then quantitative real-time RT-PCR (qRT-PCR) was carried out to compare the miR-483-5p levels in 80 pairs of ESCC tissues and adjacent non-cancerous tissues. The correlation between miR-483-5p levels and clinical features were determined.
For the TCGA cohort, ESCC patients with higher miR-483-5p had significantly shorter overall survival time. The examined ESCC cancer tissues exhibited a remarkable increment in miR-483-5p expression compared with the adjacent normal tissues. miR-483-5p was positively correlated with TNM stage, lymph nodes metastasis and T stage. In addition, upregulate miR-483-5p expression was also found to be significantly associated with poor survival of ESCC patients. Furthermore, miR-483-5p expression was an independent prognostic factor for overall survival and disease free survival in ESCC patients.
Our study demonstrates that miR-483-5p might be a tumor promoter of ESCC, which provide a promising prognostic biomarker and therapeutic target.
食管鳞状细胞癌（ESCC）是癌症相关死亡的最常见原因之一。发现能够预测ESCC发展的新型分子生物标志物将改善个性化治疗。
本研究的目的是调查miR-483-5p的表达模式并确定其在ESCC中的预后价值。
我们首先分析了从癌症基因组图谱（TCGA）队列获得的miRNA测序数据，以评估miR-483-5p在ESCC中的预后价值。然后进行定量实时RT-PCR（qRT-PCR），比较80对ESCC组织和相邻非癌组织中miR-483-5p的水平。确定miR-483-5p水平与临床特征之间的相关性。
对于TCGA队列，miR-483-5p水平较高的ESCC患者总生存时间明显较短。与相邻正常组织相比，所检测的ESCC癌组织中miR-483-5p表达显著增加。miR-483-5p与TNM分期、淋巴结转移和T分期呈正相关。此外，上调miR-483-5p表达也与ESCC患者的不良生存显著相关。此外，miR-483-5p表达是ESCC患者总生存和无病生存的独立预后因素。
我们的研究表明，miR-483-5p可能是ESCC的肿瘤促进因子，这为有前景的预后生物标志物和治疗靶点提供了依据。
