Holden H M, Meyer T E, Cusanovich M A, Daldal F, Rayment I
Department of Biochemistry, University of Arizona, Tucson 85721.
J Mol Biol. 1987 May 5;195(1):229-31. doi: 10.1016/0022-2836(87)90341-x.
Two crystal forms of the cytochrome c2 isolated from Rhodopseudomonas capsulata have been obtained. One crystal form (type I), grown from ammonium sulfate solutions at pH 7.5, belongs to the space group R32 with unit cell dimensions of a = b = 100.0 A, and c = 162.2 A in the hexagonal setting. These crystals most likely contain two molecules in the asymmetric unit. The other crystal form (type II) was obtained from polyethylene glycol 6000 solutions at pH 6.5. Type II crystals belong to the space group P3(1)21 or P3(2)21 with one molecule per asymmetric unit and unit cell dimensions of a = b = 52.4 A, and c = 87.9 A. Both crystal forms diffract to at least 1.8 A resolution and appear to be resistant to radiation damage.
已获得从荚膜红假单胞菌分离出的细胞色素c2的两种晶体形式。一种晶体形式（I型），在pH 7.5的硫酸铵溶液中生长，属于空间群R32，在六方晶系中晶胞参数为a = b = 100.0 Å，c = 162.2 Å。这些晶体的不对称单位中很可能包含两个分子。另一种晶体形式（II型）是在pH 6.5的聚乙二醇6000溶液中获得的。II型晶体属于空间群P3(1)21或P3(2)21，每个不对称单位有一个分子，晶胞参数为a = b = 52.4 Å，c = 87.9 Å。两种晶体形式的衍射分辨率至少为1.8 Å，并且似乎对辐射损伤具有抗性。
