Miki K, Saeda M, Masaki K, Kasai N, Miki M, Hayashi K
J Mol Biol. 1986 Oct 5;191(3):579-80. doi: 10.1016/0022-2836(86)90152-x.
Crystals of ferrocytochrome c2 from a non-sulphur purple photosynthetic bacterium, Rhodopseudomonas viridis, have been grown from ammonium sulphate solution at pH 8.5 by the sitting-drop vapour-diffusion procedure. The crystals belong to the trigonal system, space group P3(1)21 (or its enantiomorph P3(2)21) with unit-cell dimensions of a = b = 75.8 A and c = 40.1 A, and diffract to at least 2.0 A resolution. Assuming that an asymmetric unit contains one protein molecule (approx. 12,300 Mr), the solvent content of the crystal is approximately 54.5% (v/v).
通过坐滴气相扩散法，在pH 8.5的硫酸铵溶液中培养出了来自非硫紫色光合细菌绿假单胞菌的亚铁细胞色素c2晶体。这些晶体属于三方晶系，空间群为P3(1)21（或其对映体P3(2)21），晶胞参数为a = b = 75.8 Å，c = 40.1 Å，衍射分辨率至少为2.0 Å。假设一个不对称单位包含一个蛋白质分子（约12,300 Mr），则晶体的溶剂含量约为54.5%（v/v）。
