GP78 基因多态性与冠状动脉粥样硬化性心脏病的相关性。

Correlation between the GP78 Gene Polymorphism and Coronary Atherosclerotic Heart Disease.

机构信息

Department of Geriatrics, The First Hospital of Lanzhou University, China.

Department of Cardiovascular Medicine, Xigu Branch, Lanzhou University Second Hospital, China.

出版信息

Hellenic J Cardiol. 2018 Jan-Feb;59(1):8-13. doi: 10.1016/j.hjc.2017.02.001. Epub 2017 Feb 15.

DOI:10.1016/j.hjc.2017.02.001

PMID:28212872

Abstract

OBJECTIVE

To study the correlation between the GP78 gene polymorphism and blood fat, blood glucose, blood pressure and coronary atherosclerotic heart disease.

METHODS

A total of 72 patients with coronary atherosclerotic heart disease were selected as the observation group, and 68 healthy participants were selected as the control group. The gp78 gene polymorphism of both groups was studied via polymerase chain reaction-restriction fragment length polymorphism (RFLP). At the same time, the multiple expression quantities of the GP78 gene in the tissues of both groups were tested via fluorogenic quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and Western-blotting assay. Furthermore, the blood fat, blood glucose and blood pressure of subjects in both groups were tested.

RESULTS

The percentages of the gp78 gene polymorphisms of Arg/Arg, Arg/Gly and Gly/Gly at the 145 locus of the study subjects in the observation group were 12.3%, 43.2% and 44.5%, respectively, while those in the control group were 74.3%, 11.2% and 14.5%, respectively, and there were significant differences between both groups. Based on the test results of the blood fat, blood glucose and blood pressure of the objects in the observation group and control group, significant differences were found between the two groups (P<0.05).

CONCLUSION

There was a significant correlation between the 145 locus of the gp89 gene and coronary atherosclerotic heart disease, indexes of blood fat, blood glucose and blood pressure.

摘要

目的

研究 GP78 基因多态性与血脂、血糖、血压及冠状动脉粥样硬化性心脏病的相关性。

方法

选取 72 例冠状动脉粥样硬化性心脏病患者作为观察组，选取同期 68 例健康体检者作为对照组。采用聚合酶链反应-限制性片段长度多态性（RFLP）方法对两组 gp78 基因多态性进行研究。同时采用荧光定量 PCR、酶联免疫吸附试验（ELISA）、Western-blotting 法检测两组组织中 GP78 基因的多重表达量，检测两组受检者的血脂、血糖、血压。

结果

观察组研究对象 145 位 gp78 基因多态性 Arg/Arg、Arg/Gly、Gly/Gly 比例分别为 12.3%、43.2%、44.5%，对照组分别为 74.3%、11.2%、14.5%，两组比较差异有统计学意义。观察组和对照组受检者的血脂、血糖、血压检测结果比较，差异均有统计学意义（P<0.05）。