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利用来自埃及的小核糖体RNA对独特的附肢中腹盘吸虫(吸虫纲:杯殖科)进行分子特征分析。

Molecular characterization of the unique Mesostephanus appendiculatus (Trematoda: Cyathocotylidae) by small ribosomal RNA from Egypt.

作者信息

El-Bahy Nasr M, Bazh Eman K, Sorour Shimaa S, Elhawary Nagwa M

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, Sadat City University, Sadat, Egypt.

Department of Pathology & Parasitology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.

出版信息

Parasitol Res. 2017 Apr;116(4):1129-1136. doi: 10.1007/s00436-016-5342-5. Epub 2017 Feb 18.

DOI:10.1007/s00436-016-5342-5
PMID:28213655
Abstract

M esostephanus appendiculatus (Family: Cyathocotylidae) is one of the unique trematodes that complete their cycles in human and animal intestines in many countries of the world. The main source of its transmission is eating raw or undercooked infected fish muscle. Earliest analyses of genes to different parasites supported the analysis of helminthes either biological or morphological. This paper detected M. appendiculatus sequence with GenBank accession number gb (KY026782). Comparison of M. appendiculatus with other helminthes using BioEdit 7 and MEGA7 program shows some similarity in different points along its sequence. The phylogenetic analysis clarifies that it was closely related to both trematodes (Clinostomum complanatum and Echinochasmus japonicus) and some cestodes of fish origin such as Polyonchobothrium polypteri, Bothriocephalus sp., and Haplobothrium globuliforme. The obtained results provide a good source for genome analysis of M. appendiculatus in relation to other Platyhelminthes.

摘要

阑尾中睾吸虫(科:杯殖科)是世界上许多国家在人类和动物肠道内完成其生命周期的独特吸虫之一。其传播的主要来源是食用生的或未煮熟的受感染鱼肉。对不同寄生虫的基因进行的最早分析支持了对蠕虫的生物学或形态学分析。本文检测到的阑尾中睾吸虫序列的GenBank登录号为gb(KY026782)。使用BioEdit 7和MEGA7程序将阑尾中睾吸虫与其他蠕虫进行比较,结果显示其序列在不同点上存在一些相似性。系统发育分析表明,它与吸虫(扁平嗜口吸虫和日本棘隙吸虫)以及一些鱼类来源的绦虫,如多鳍鱼多槽绦虫、裂头绦虫属和球形单槽绦虫密切相关。所获得的结果为阑尾中睾吸虫与其他扁形动物的基因组分析提供了良好的来源。

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