Mazurenko N N, Gladkova T I, Mikhaleva I I, Antonenko V V, Prudchenko I A
Mol Biol (Mosk). 1987 Jul-Aug;21(4):972-80.
To generate the antibodies to the transforming protein (p28sis) of simian sarcoma virus (SSV), the rabbits were immunized with peptides, corresponding to 200-206 and 201-210 sequences of p28sis, conjugated with protein carriers by different ways. The synthesis of peptides was carried out by the classical techniques in solution by using the benzyl type side protecting groups. Antibody titres against peptides were determined by ELISA and protein specificity by radioimmunoprecipitation and immunoblotting. It was shown that the antibodies to 201-210 peptide recognize p28sis and its dimer p56sis in marmoset and rat cells transformed by simian sarcoma virus.
为了产生针对猿猴肉瘤病毒（SSV）转化蛋白（p28sis）的抗体，用与p28sis的200 - 206和201 - 210序列相对应的肽，通过不同方式与蛋白质载体偶联，对兔子进行免疫。肽的合成采用经典的溶液技术，使用苄基型侧链保护基团。通过酶联免疫吸附测定（ELISA）测定针对肽的抗体效价，通过放射免疫沉淀和免疫印迹测定蛋白质特异性。结果表明，针对201 - 210肽的抗体能够识别在猿猴肉瘤病毒转化的狨猴和大鼠细胞中的p28sis及其二聚体p56sis。
