Cordeiro Cynthia A, Vieira Erica L M, Castro Vinicius M, Dutra Walderez O, Costa Rogerio A, Orefice Juliana L, Campos Wesley R, Orefice Fernando, Young Lucy H, Teixeira Antonio Lucio
Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil; Centro Brasileiro de Ciências Visuais, Belo Horizonte, MG, Brazil; Retina Department, Massachusetts Eye and Ear Infirmary, Boston, MA, USA.
Interdisciplinary Laboratory of Medical Investigation, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Immunol Lett. 2017 Apr;184:84-91. doi: 10.1016/j.imlet.2017.02.009. Epub 2017 Feb 16.
Toxoplasma gondii infection is an important cause of infectious ocular disease. The physiopathology of retinochoroidal lesions associated with this infection is not completely understood. The present study was undertaken to investigate cytokine production by T cells from individuals with active toxoplasmic retinochoroiditis (TR) comparing with controls. Eighteen patients with active TR and 15 healthy controls (6 controls IgG to Toxoplasma and 9 negative controls) were included in the study. Peripheral blood mononuclear cells were incubated in the presence or absence of T. gondii antigen (STAg), and stained against CD4, CD8, TNF, IL-10 and IFN-γ. Baseline expression of cytokines was higher in TR/IgG patients in comparison with controls. Cytokine expression was not increased by STAg in vitro stimulation in controls. After stimulation, TR/IgG patients' lymphocytes increased cytokine as compared to cultures from both controls. While T cells were the main source of IL-10, but also IFN-γ and TNF, other lymphocyte populations were relevant source of inflammatory cytokines. Interestingly, it was observed a negative correlation between ocular lesion size and IL-10 expression by CD4 lymphocytes. This study showed that T cells are the main lymphocyte populations expressing IL-10 in patients with TR. Moreover, expression of IL-10 plays a protective role in active TR.
弓形虫感染是感染性眼病的一个重要病因。与这种感染相关的视网膜脉络膜病变的生理病理学尚未完全明确。本研究旨在调查活动性弓形虫视网膜脉络膜炎（TR）患者与对照组相比T细胞产生细胞因子的情况。18例活动性TR患者和15名健康对照者（6名弓形虫IgG阳性对照和9名阴性对照）纳入研究。外周血单个核细胞在有或无弓形虫抗原（STAg）存在的情况下孵育，并针对CD4、CD8、TNF、IL-10和IFN-γ进行染色。与对照组相比，TR/IgG患者细胞因子的基线表达更高。在对照组中，STAg体外刺激未增加细胞因子表达。刺激后，与两个对照组的培养物相比，TR/IgG患者的淋巴细胞增加了细胞因子的产生。虽然T细胞是IL-10的主要来源，但也是IFN-γ和TNF的主要来源，其他淋巴细胞群体也是炎性细胞因子的相关来源。有趣的是，观察到眼部病变大小与CD4淋巴细胞IL-10表达之间呈负相关。本研究表明，T细胞是TR患者中表达IL-10的主要淋巴细胞群体。此外，IL-10的表达在活动性TR中起保护作用。
