Fatoohi Fatih, Cozon Grégoire Jacques Noël, Wallon Martine, Kodjikian Laurent, Peyron François
E. A. 3087 Parasitologie, Faculté de Médecine, Lyon Nord, Université Cl. Bernard Lyon1, France.
Jpn J Ophthalmol. 2006 Mar-Apr;50(2):103-10. doi: 10.1007/s10384-005-0295-8.
Analysis of systemic cellular response to Toxoplasma antigen in patients with ocular toxoplasmosis.
Activated (CD25(+)) T cells were detected by flow cytometry after a 7-day culture of whole blood from patients with ocular (n = 16) or asymptomatic (n = 14) toxoplasmosis, and controls (n = 10), in the presence of soluble Toxoplasma antigen (ST-Ag). Interferon (IFN)-gamma, interleukin (IL) 4, and IL-10 were measured in culture supernatants by enzyme-linked immunosorbent assay.
Higher percentages of CD25(+) T cells were detected in ST-Ag-activated cultures from Toxoplasma-infected patients, with or without ocular lesions (37.0 +/- 19.1% or 41.1 +/- 19.3%, respectively) than from controls (3.2 +/- 1.2%) (P < 0.0001). Differences were not statistically significant between asymptomatic and ocular toxoplasmosis (P > 0.4) or among congenital, acquired, and undetermined ocular toxoplasmosis (P > 0.2). Higher levels of IFN-gamma were detected in ST-Ag-stimulated blood cultures from infected patients than in those from controls (P < 0.0001), with no difference between patients with asymptomatic or ocular toxoplasmosis (P > 0.05). IL-10 was detected only in activated culture supernatants from three patients with ocular toxoplasmosis and two patients with asymptomatic toxoplasmosis. IL-4 was never produced in ST-Ag-activated cultures.
Systemic cellular response to ST-Ag does not differ between the patients with ocular and asymptomatic toxoplasmosis with regard to activation markers and type 1 cytokine production.
分析眼部弓形虫病患者对弓形虫抗原的全身细胞反应。
将眼部弓形虫病患者（n = 16）、无症状弓形虫病患者（n = 14）及对照组（n = 10）的全血在可溶性弓形虫抗原（ST-Ag）存在的情况下培养7天，然后通过流式细胞术检测活化的（CD25(+)）T细胞。通过酶联免疫吸附测定法测量培养上清液中的干扰素（IFN）-γ、白细胞介素（IL）4和IL-10。
在有或无眼部病变的弓形虫感染患者的ST-Ag活化培养物中检测到的CD25(+) T细胞百分比（分别为37.0 +/- 19.1%或41.1 +/- 19.3%）高于对照组（3.2 +/- 1.2%）（P < 0.0001）。无症状弓形虫病和眼部弓形虫病之间（P > 0.4）或先天性、获得性和未确诊的眼部弓形虫病之间（P > 0.2）差异无统计学意义。在感染患者的ST-Ag刺激的血液培养物中检测到的IFN-γ水平高于对照组（P < 0.0001），无症状或眼部弓形虫病患者之间无差异（P > 0.05）。仅在3例眼部弓形虫病患者和2例无症状弓形虫病患者的活化培养上清液中检测到IL-10。在ST-Ag活化培养物中从未产生IL-4。
眼部和无症状弓形虫病患者对ST-Ag的全身细胞反应在活化标志物和1型细胞因子产生方面无差异。
