Liu Luhao, Li Ermao, Li Futian, Luo Lianmin, Zhao Shankun, Kang Ran, Luo Jintai, Zhao Zhigang
Department of Urology and Andrology, Minimally Invasive Surgery Center, Guangdong Provincial Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University. Guangzhou, Guangdong, China.
Department of Urology and Andrology, Minimally Invasive Surgery Center, Guangdong Provincial Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University. Guangzhou, Guangdong, China.
Urology. 2017 May;103:273.e1-273.e6. doi: 10.1016/j.urology.2017.02.020. Epub 2017 Feb 15.
To investigate the effect of testosterone (T) on the phenotypic modulation of corpus cavernosum smooth muscle (CCSM) cells in a castrated rat model.
Thirty male Sprague-Dawley rats were randomly divided into 3 groups: control, castration, and castration with T supplementation (castration + T). Erectile function, histologic change, and biochemical markers were assessed for phenotypic modulation of CCSM cells in corporal tissue. Moreover, the primary rat CCSM cells were isolated and examined by Western blot analysis.
Our data showed that serum T level, mean weight of the body, erectile function, and smooth muscle-to-collagen ratio were significantly decreased in the castration group compared with those in the control and castration + T groups. The expressions of CCSM cells' phenotypic markers, such as α-smooth muscle actin, calponin, and smooth muscle myosin heavy chain 11, were markedly lower, whereas osteopontin protein expression was significantly higher in castrated rats than in control and castrated + T rats. In addition, the immunofluorescence staining of α-smooth muscle actin and calponin markedly decreased in the primary CCSM cells of the castrated rats compared with the intensity of the control and the castration + T rats.
CCSM cells undergo phenotype modulation in castrated rats, whereas T reversed the alterations. T may play a key role in the phenotype modulation of CCSM cells.
在去势大鼠模型中研究睾酮（T）对阴茎海绵体平滑肌（CCSM）细胞表型调节的影响。
30只雄性Sprague-Dawley大鼠随机分为3组：对照组、去势组和去势补充T组（去势+T）。评估阴茎组织中CCSM细胞表型调节的勃起功能、组织学变化和生化标志物。此外，分离原代大鼠CCSM细胞并通过蛋白质免疫印迹分析进行检测。
我们的数据显示，与对照组和去势+T组相比，去势组的血清T水平、平均体重、勃起功能和平滑肌与胶原蛋白比例显著降低。去势大鼠中CCSM细胞表型标志物如α-平滑肌肌动蛋白、钙调蛋白和平滑肌肌球蛋白重链11的表达明显较低，而骨桥蛋白的蛋白表达显著高于对照组和去势+T大鼠。此外，与对照组和去势+T大鼠相比，去势大鼠原代CCSM细胞中α-平滑肌肌动蛋白和钙调蛋白的免疫荧光染色明显减弱。
去势大鼠的CCSM细胞发生表型调节，而T可逆转这些改变。T可能在CCSM细胞的表型调节中起关键作用。
