De Koning P, Gispen W H
Division of Molecular Neurobiology, Rudolf Magnus Institute for Pharmacology, University of Utrecht, The Netherlands.
Peptides. 1987 May-Jun;8(3):415-22. doi: 10.1016/0196-9781(87)90003-9.
The beneficial effect of short-term (8 days) melanocortin therapy on regenerating peripheral nerves is demonstrated using functional and electrophysiological tests. Following a crush lesion of the rat sciatic nerve, recovery of sensory function is monitored by assessing the responsiveness of the rat to a small electric current applied to the footsole. Recovery of motor function is assessed by means of an analysis of walking patterns. Normalization of the walking pattern reflects reinnervation of different muscle groups. The motor and H-reflex related sensory nerve conduction velocity of the regenerated nerves are longitudinally investigated in the same rats in which the recovery of motor and sensory function had been assessed previously. Functional tests show an enhanced recovery under melanocortin therapy, but in the end both saline- and melanocortin-treated rats show 100% recovery. However, when compared to the contralateral sciatic nerve, in the peptide-treated animals motor nerve conduction in the regenerated nerves has fully recovered after about 90 days following the crush lesion and the sensory conduction after about 120 days, whereas in the saline-treated rats a deficit of 20-40% in both motor and sensory conduction remains. This difference is observed even 214 days following crush.
通过功能和电生理测试证明了短期（8天）黑素皮质素疗法对再生周围神经的有益作用。在大鼠坐骨神经挤压损伤后，通过评估大鼠对施加于足底的小电流的反应性来监测感觉功能的恢复。通过分析行走模式来评估运动功能的恢复。行走模式的正常化反映了不同肌肉群的再支配。在先前评估了运动和感觉功能恢复的同一批大鼠中，纵向研究再生神经的运动和与H反射相关的感觉神经传导速度。功能测试表明，黑素皮质素疗法可促进恢复，但最终生理盐水处理组和黑素皮质素处理组的大鼠均显示100%恢复。然而，与对侧坐骨神经相比，在肽处理的动物中，再生神经的运动神经传导在挤压损伤后约90天完全恢复，感觉传导在约120天后完全恢复，而在生理盐水处理的大鼠中，运动和感觉传导均仍有20-40%的缺陷。即使在挤压后214天仍观察到这种差异。
