Aversano Riccardo, Contaldi Felice, Adelfi Maria Grazia, D'Amelia Vincenzo, Diretto Gianfranco, De Tommasi Nunziatina, Vaccaro Carmen, Vassallo Antonio, Carputo Domenico
Department of Agricultural Sciences, University of Naples Federico II, Portici 80055, Italy.
Italian National Agency for New Technologies, Energy, and Sustainable Development, Casaccia Research Centre, Rome 00123, Italy.
Phytochemistry. 2017 May;137:42-51. doi: 10.1016/j.phytochem.2017.02.011. Epub 2017 Feb 16.
The cultivated potato Solanum tuberosum is unrivalled among crop plants for its wild relatives, which potentially represent an important source of genetic diversity to improve the nutritional value of potato varieties and understand metabolism regulation. The main aim of this research was to profile human health-related metabolites in a number of clones from 13 Solanum species. Results from HPLC-DAD and LC-ESI-MS analyses highlighted a high interspecific variability in the level of metabolites analysed. Ascorbic acid was confirmed to be the most abundant antioxidant in potato and chlorogenic acid the primary polyphenol. Generally, metabolite-based hierarchical clustering (HCL) and correlation networks did not group clones of identical species in the same cluster. This might be due to various factors, including the outcrossing nature of potato species, gene expression level and metabolic profiling techniques. Access to the genome sequence of S. tuberosum and S. commersonii allowed comparison of the genes involved in ascorbic acid, aromatic amino acid, phenylpropanoid and glycoalkaloid biosynthesis and helped interpret their respective pathways.
栽培马铃薯（Solanum tuberosum）在作物中无与伦比，因为它拥有众多野生近缘种，这些野生近缘种可能是提高马铃薯品种营养价值和理解代谢调控的重要遗传多样性来源。本研究的主要目的是分析13种茄属植物多个克隆中与人类健康相关的代谢物。高效液相色谱-二极管阵列检测法（HPLC-DAD）和液相色谱-电喷雾电离质谱分析法（LC-ESI-MS）的结果突出显示了所分析代谢物水平存在高度种间变异性。已证实抗坏血酸是马铃薯中含量最丰富的抗氧化剂，而绿原酸是主要的多酚类物质。一般来说，基于代谢物的层次聚类（HCL）和相关网络并未将同一物种的克隆归为同一聚类。这可能是由于多种因素造成的，包括马铃薯物种的异交特性、基因表达水平和代谢物分析技术。马铃薯（S. tuberosum）和康氏茄（S. commersonii）基因组序列的获取使得对参与抗坏血酸、芳香族氨基酸、苯丙烷类化合物和糖苷生物碱生物合成的基因进行比较成为可能，并有助于解释它们各自的途径。
