Zhang Haijuan, Qi Hexing, Lu Guangxin, Zhou Xueli, Wang Junbang, Li Jingjing, Zheng Kaifu, Fan Yuejun, Zhou Huakun, Wang Jiuluan, Wu Chu
College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.
Experimental Station of Grassland Improvement of Qinghai Province, Xining, China.
Front Microbiol. 2023 Aug 7;14:1134585. doi: 10.3389/fmicb.2023.1134585. eCollection 2023.
is a perennial grass of the Gramineae family. Due to its cold-resistance and nutrition deficiency tolerance, it has been applied to the ecological restoration of degraded alpine grassland on the Qinghai-Tibet Plateau. As an important symbiotic microorganism, arbuscular mycorrhizal fungi (AMF) have been proven to have great potential in promoting the growth and stress resistance of Gramineae grasses. However, the response mechanism of the AMF needs to be clarified. Therefore, in this study, was used to explore the mechanism regulating cold resistance of . Based on pot experiments and metabolomics, the effects of were investigated on the activities of antioxidant enzyme and metabolites in the roots of under cold stress (15/10°C, 16/8 h, day/night). The results showed that lipids and lipid molecules are the highest proportion of metabolites, accounting for 14.26% of the total metabolites. The inoculation with had no significant effects on the activities of antioxidant enzyme in the roots of at room temperature. However, it can significantly change the levels of some lipids and other metabolites in the roots. Under cold stress, the antioxidant enzyme activities and the levels of some metabolites in the roots of were significantly changed. Meanwhile, most of these metabolites were enriched in the pathways related to plant metabolism. According to the correlation analysis, the activities of antioxidant enzyme were closely related to the levels of some metabolites, such as flavonoids and lipids. In conclusion, AMF may regulate the cold-resistance of Gramineae grasses by affecting plant metabolism, antioxidant enzyme activities and antioxidant-related metabolites like flavonoids and lipids. These results can provide some basis for studying the molecular mechanism of AMF regulating stress resistance of Gramineae grasses.
是禾本科的一种多年生草本植物。由于其抗寒性和耐营养缺乏性，已被应用于青藏高原退化高寒草地的生态恢复。丛枝菌根真菌（AMF）作为一种重要的共生微生物，已被证明在促进禾本科草类生长和抗逆性方面具有巨大潜力。然而，AMF的响应机制尚需阐明。因此，本研究利用[具体研究对象]来探究[具体植物名称]抗寒调控机制。基于盆栽试验和代谢组学，研究了[具体内容]对[具体植物名称]在冷胁迫（15/10°C，16/8小时，昼/夜）下根系抗氧化酶活性和代谢产物的影响。结果表明，脂质和脂质分子是代谢产物中占比最高的，占总代谢产物的14.26%。在室温下接种[具体内容]对[具体植物名称]根系抗氧化酶活性无显著影响。然而，它能显著改变根系中一些脂质和其他代谢产物的水平。在冷胁迫下，[具体植物名称]根系的抗氧化酶活性和一些代谢产物水平发生显著变化。同时，这些代谢产物大多富集在与植物代谢相关的途径中。根据相关性分析，抗氧化酶活性与一些代谢产物水平密切相关，如黄酮类化合物和脂质。综上所述，AMF可能通过影响植物代谢、抗氧化酶活性以及黄酮类化合物和脂质等抗氧化相关代谢产物来调节禾本科草类的抗寒性。这些结果可为研究AMF调控禾本科草类抗逆性的分子机制提供一定依据。
