Rezaei Marzieh, Hosseini Ahmad, Nikeghbalian Saman, Ghaderi Abbas
Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Pancreatology. 2017 Mar-Apr;17(2):303-309. doi: 10.1016/j.pan.2017.02.003. Epub 2017 Feb 8.
Basic research in the field of acinar cell carcinoma (ACC) as a rare neoplasm of the pancreas is dependent on the availability of pragmatic model such as new pancreatic cancer cell lines. Thus, establishment and characterization of new pancreatic cancer cell lines from ACC origin are deemed important.
Faraz-ICR cell line was derived from a 58-years old woman with pancreatic acinar cell carcinoma by the collagenase digestion protocol. We characterized the cell line by examining its morphology and cytostructural and functional profile.
Faraz-ICR has a doubling time of 35 hours and grows in soft agar with a colony-forming efficiency of 25%. The cell had nearly normal pattern of chromosomes in karyotype analysis and Comparative Genomic Hybridization (CGH) array analysis. Evaluation of cells by flowcytometry showed that Faraz-ICR is negative for EpCAM and mesenchymal markers in different passages, and has epithelial nature. Immunofluorescence staining revealed that cells were strongly positive for vimentin, desmin, ezrin, S100, nestin and they were negative for pan-cytokeratins, chromogranin and alpha smooth muscle actin.
We were able to establish a new pancreatic carcinoma cell line with partial aspects of Epithelial-mesenchymal transition and aggressiveness. This cell line might be suitable for studying various anticancer drugs and protein profile aiming to see any possible tumor associated marker for ACC.
胰腺腺泡细胞癌（ACC）作为一种罕见的胰腺肿瘤，其基础研究依赖于实用模型的可用性，如新的胰腺癌细胞系。因此，建立并鉴定源自ACC的新胰腺癌细胞系被认为很重要。
通过胶原酶消化法从一名58岁患有胰腺腺泡细胞癌的女性患者中获得了Faraz-ICR细胞系。我们通过检查其形态、细胞结构和功能特征来鉴定该细胞系。
Faraz-ICR的倍增时间为35小时，能在软琼脂中生长，集落形成效率为25%。在核型分析和比较基因组杂交（CGH）阵列分析中，该细胞的染色体模式近乎正常。通过流式细胞术对细胞进行评估显示，Faraz-ICR在不同传代中上皮细胞黏附分子（EpCAM）和间充质标志物呈阴性，具有上皮性质。免疫荧光染色显示，细胞波形蛋白、结蛋白、埃兹蛋白、S100、巢蛋白呈强阳性，而泛细胞角蛋白、嗜铬粒蛋白和α平滑肌肌动蛋白呈阴性。
我们成功建立了一种具有部分上皮-间质转化和侵袭性特征的新胰腺癌细胞系。该细胞系可能适合用于研究各种抗癌药物和蛋白质谱，以寻找ACC可能的肿瘤相关标志物。
