Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Research Center of Otolaryngology Head and Neck Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.
Asian Pac J Cancer Prev. 2021 Jan 1;22(1):99-103. doi: 10.31557/APJCP.2021.22.1.99.
Tongue cancer is one of the most aggressive forms of oral squamous cell carcinoma which needs more investigations. Herein, we aimed to establish and characterize a tongue cancer cell line.
Tumor tissue was obtained from a 70-year-old woman with tongue cancer. The established cell line named as EZB-ICR and characterized for doubling time, expression of specific markers, HPV corporation and migration status using flow cytometry, immunofluorescence staining, multiplex PCR, and migration assay.
EZB-ICR was negative for expression of mesenchymal specific markers, cytokeratin19, pan-cytokeratin, vimentin and EPCAM, but was positive for S100 and Nestin. No appearance of human papilloma virus DNA was seen. The doubling time of EZB-ICR was 31 hours and migration assay confirmed its metastatic nature.
To the best of our knowledge, EZB-ICR is the first tongue human cancer cell line established in Iran, and its features make it appropriate for cancer-based in vitro studies and contribute to more studies on tongue cancer.
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舌癌是口腔鳞状细胞癌中最具侵袭性的形式之一，需要更多的研究。在此，我们旨在建立和鉴定一种舌癌细胞系。
从一名 70 岁的舌癌女性患者中获得肿瘤组织。建立的细胞系命名为 EZB-ICR，并通过流式细胞术、免疫荧光染色、多重 PCR 和迁移实验，对其倍增时间、特定标志物的表达、HPV 合作和迁移状态进行了表征。
EZB-ICR 对间充质特异性标志物、细胞角蛋白 19、泛细胞角蛋白、波形蛋白和 EPCAM 的表达呈阴性，但对 S100 和 Nestin 呈阳性。未发现人乳头瘤病毒 DNA。EZB-ICR 的倍增时间为 31 小时，迁移实验证实其具有转移性。
据我们所知，EZB-ICR 是伊朗建立的第一个人舌癌细胞系，其特征使其适合基于癌症的体外研究，并有助于更多的舌癌研究。
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