College of Natural Resources and Environment, Agriculture Key Laboratory of Plant Nutrition and Agri-Environment in Northwest China, Northwest A&F University, Yangling 712100, China.
Int J Environ Res Public Health. 2018 Apr 10;15(4):708. doi: 10.3390/ijerph15040708.
The spread of antibiotic resistance genes in river systems is an emerging environmental issue due to their potential threat to aquatic ecosystems and public health. In this study, we used droplet digital polymerase chain reaction (ddPCR) to evaluate pollution with clinically relevant antibiotic resistance genes (ARGs) at 13 monitoring sites along the main stream of the Weihe River in China. Six clinically relevant ARGs and a class I integron-integrase () gene were analyzed using ddPCR, and the bacterial community was evaluated based on the bacterial 16S rRNA V3-V4 regions using MiSeq sequencing. The results indicated Proteobacteria, Actinobacteria, Cyanobacteria, and Bacteroidetes as the dominant phyla in the water samples from the Weihe River. Higher abundances of , , , and genes (10³ to 10⁵ copies/mL) were detected in the surface water samples compared with the relatively low abundances of , , and genes (0-1.94 copies/mL). Eight bacterial genera were identified as possible hosts of the gene and three ARGs (, , and ) based on network analysis. The results suggested that the bacterial community structure and horizontal gene transfer were associated with the variations in ARGs.
抗生素耐药基因在水系中的传播是一个新出现的环境问题，因为它们对水生生态系统和公众健康构成潜在威胁。在这项研究中，我们使用液滴数字聚合酶链反应（ddPCR）在沿中国渭河干流的 13 个监测点评估了临床上相关的抗生素耐药基因（ARGs）的污染情况。使用 ddPCR 分析了 6 种临床上相关的 ARGs 和一个 I 类整合子整合酶（）基因，并通过 MiSeq 测序基于细菌 16S rRNA V3-V4 区评估了细菌群落。结果表明，变形菌门、放线菌门、蓝细菌门和拟杆菌门是渭河水样中的主要门。与相对较低的 、 、和 基因丰度（0-1.94 拷贝/mL）相比，地表水样品中 、 、 和 基因的丰度更高（10³ 到 10⁵ 拷贝/mL）。基于网络分析，确定了 8 个可能是 基因和 3 个 ARGs（、和 ）宿主的细菌属。结果表明，细菌群落结构和水平基因转移与 ARGs 的变化有关。
