Lei Xuan, Zhang Cong, Jiang Zhibo, Li Xingxing, Shi Yuanyuan, Liu Ming, Xie Yunying, Wang Lifei, Hong Bin
Key Laboratory of Biotechnology of Antibiotics, The National Health and Family Planning Commission (NHFPC), Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Immunology Program Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
J Biotechnol. 2017 Apr 10;247:6-10. doi: 10.1016/j.jbiotec.2017.02.013. Epub 2017 Feb 16.
Amycolatopsis orientalis CPCC200066 is an actinomycete exploited commercially in China for the production of norvancomycin, an important glycopeptide antibiotic structurally close to the well-known vancomycin. The availability of the complete genome sequence of CPCC200066 would greatly strengthen our understanding of the regulation pattern of norvancomycin biosynthesis and ultimately improve its production, as well as potentiate discoveries of novel bioactive compounds. Here we report the complete genome sequence of A. orientalis CPCC200066, a circular chromosome consisting of 9,490,992bp. Forty putative secondary metabolite biosynthetic gene clusters, including norvancomycin, were predicted, covering 20.3% of the whole genome. To facilitate genetic manipulation of this strain, an efficient transformation system was established by constructing a novel integrative vector pIMBT1, which could be transferred into CPCC200066 by electroporation with high efficiency. ΦBT1 attB sites were also identified in other known Amycolatopsis genomes, indicating pIMBT1's prospect to be a novel vector for genus Amycolatopsis.
东方拟无枝酸菌CPCC200066是一种放线菌，在中国被用于商业生产去甲万古霉素，这是一种重要的糖肽类抗生素，其结构与著名的万古霉素相近。CPCC200066完整基因组序列的可得性将极大地增强我们对去甲万古霉素生物合成调控模式的理解，并最终提高其产量，同时也有助于发现新型生物活性化合物。在此，我们报道了东方拟无枝酸菌CPCC200066的完整基因组序列，它是一条由9,490,992bp组成的环状染色体。预测了包括去甲万古霉素在内的40个假定的次生代谢物生物合成基因簇，覆盖了整个基因组的20.3%。为便于对该菌株进行遗传操作，通过构建新型整合载体pIMBT1建立了高效转化系统，该载体可通过电穿孔高效转入CPCC200066。在其他已知的拟无枝酸菌基因组中也鉴定到了ΦBT1 attB位点，这表明pIMBT1有望成为拟无枝酸菌属的新型载体。
