Chou H S, Nelson C A, Godambe S A, Chaplin D D, Loh D Y
Howard Hughes Medical Institute, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Science. 1987 Oct 23;238(4826):545-8. doi: 10.1126/science.2821625.
The complete germline organization of the beta-chain genes of the murine T cell receptor was elucidated in order to obtain the structural basis for understanding the mechanisms of somatic DNA rearrangements. Twenty of the 22 known variable (V beta) genes are clustered within 250 kilobases of DNA 5' to the constant region (C beta) genes. These V beta genes share the same transcriptional orientation as the diversity (D beta), joining (J beta), and C beta genes, which implies that chromosomal deletion is the mechanism for most V beta to D beta-J beta rearrangements. Within this V beta cluster, the distance between the most proximal V beta gene and the D beta-J beta-C beta cluster is 320 kilobases, as determined by field-inversion gel electrophoresis. The large distance between V beta and D beta, relative to that between D beta and J beta, may have significant implications for the ordered rearrangement of the T cell receptor beta-chain genes.
为了获得理解体细胞DNA重排机制的结构基础，对小鼠T细胞受体β链基因的完整种系组织进行了阐明。22个已知可变（Vβ）基因中的20个聚集在恒定区（Cβ）基因5'端250千碱基的DNA范围内。这些Vβ基因与多样性（Dβ）、连接（Jβ）和Cβ基因具有相同的转录方向，这意味着染色体缺失是大多数Vβ到Dβ-Jβ重排的机制。通过场反转凝胶电泳确定，在这个Vβ簇内，最靠近的Vβ基因与Dβ-Jβ-Cβ簇之间的距离为320千碱基。相对于Dβ和Jβ之间的距离，Vβ和Dβ之间的大距离可能对T细胞受体β链基因的有序重排具有重要意义。
