小鼠Igh-V基因座的组织。VH基因家族簇的分散、散布及进化。
The organization of the mouse Igh-V locus. Dispersion, interspersion, and the evolution of VH gene family clusters.
作者信息
Brodeur P H, Osman G E, Mackle J J, Lalor T M
机构信息
Immunology Program, Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts.
出版信息
J Exp Med. 1988 Dec 1;168(6):2261-78. doi: 10.1084/jem.168.6.2261.
We have constructed a panel of Abelson murine leukemia virus-transformed pre-B cells to study the organization of the mouse VH gene families. Based on the analyses of VH gene deletions on 51 chromosomes with VH gene rearrangements, we have inferred a map order of the Igh locus that holds for both the Igha and Ighb haplotypes. We show that members of each VH gene family are generally clustered, although three family clusters (VHS107, VH36-60, VGAM3.8) are dispersed in two or three subregions of the locus. Members of two VH gene families, VHQ52 and VH7183, are extensively interspersed and map within the same subregion. An examination of the distribution of VH group members (VH II, I, and III) within the locus suggests that two major duplications may, in part, explain the dispersed pattern of VH family clusters. The relationship of VH organization and functional expression is discussed in terms of position-dependent and complexity-driven models.
我们构建了一组阿贝尔逊鼠白血病病毒转化的前B细胞，以研究小鼠VH基因家族的组织方式。基于对51条发生VH基因重排的染色体上VH基因缺失的分析，我们推断出了Igh位点的图谱顺序，该顺序适用于Igha和Ighb单倍型。我们发现，每个VH基因家族的成员通常是聚集的，尽管三个家族簇（VHS107、VH36 - 60、VGAM3.8）分散在该位点的两个或三个亚区域中。两个VH基因家族VHQ52和VH7183的成员广泛散布，并定位在同一亚区域内。对该位点内VH组成员（VH II、I和III）分布的研究表明，两个主要的重复可能部分解释了VH家族簇的分散模式。我们从位置依赖性和复杂性驱动模型的角度讨论了VH组织与功能表达之间的关系。
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