Fu Yiqian, van Silfhout Alex, Shahin Arwa, Egberts Ronny, Beers Martin, van der Velde Ans, van Houten Adrie, van Tuyl Jaap M, Visser Richard G F, Arens Paul
Plant Breeding, Wageningen University & Research, PO Box 386, 6700AJ Wageningen, The Netherlands.
Schreurs Holland B.V., Hoofdweg 81, 1424PD De Kwakel, The Netherlands.
Mol Breed. 2017;37(2):13. doi: 10.1007/s11032-016-0617-1. Epub 2017 Jan 23.
is an economically important cut flower. In the production and transportation of gerbera with unavoidable periods of high relative humidity, grey mould occurs and results in losses in quality and quantity of flowers. Considering the limitations of chemical use in greenhouses and the impossibility to use these chemicals in auction or after sale, breeding for resistant gerbera cultivars is considered as the best practical approach. In this study, we developed two segregating F1 populations (called S and F). Four parental linkage maps were constructed using common and parental specific SNP markers developed from expressed sequence tag sequencing. Parental genetic maps, containing 30, 29, 27 and 28 linkage groups and a consensus map covering 24 of the 25 expected chromosomes, could be constructed. After evaluation of disease severity using three different tests, , (of disc florets) and , quantitative trait locus (QTL) mapping was performed using the four individual parental maps. A total of 20 QTLs (including one identical QTL for whole inflorescence and bottom tests) were identified in the parental maps of the two populations. The number of QTLs found and the explained variance of most QTLs detected reflect the complex mechanism of disease response.
是一种具有重要经济价值的切花。在非洲菊的生产和运输过程中，不可避免地会出现相对湿度较高的时期，此时会发生灰霉病，导致花朵的质量和数量受损。考虑到温室中化学药剂使用的局限性以及在拍卖或售后无法使用这些化学药剂的情况，培育抗灰霉病的非洲菊品种被认为是最佳的实际方法。在本研究中，我们构建了两个分离的F1群体（称为S和F）。利用从表达序列标签测序开发的通用和亲本特异性单核苷酸多态性（SNP）标记构建了四张亲本连锁图谱。可以构建分别包含30、29、27和28个连锁群的亲本遗传图谱以及覆盖25条预期染色体中24条的共识图谱。在使用三种不同测试评估病害严重程度后，即 、 （舌状花的）和 ，利用四张单独的亲本图谱进行了数量性状位点（QTL）定位。在两个群体的亲本图谱中总共鉴定出20个QTL（包括一个用于整个花序和底部测试的相同QTL）。所发现的QTL数量以及大多数检测到的QTL所解释的变异反映了对灰霉病反应的复杂机制。
