Biology Department, Centre for Novel Agricultural Products (CNAP), University of York, Wentworth Way, York, YO10 5DD, UK.
Department of Agriculture and Environment, Harper Adams University, Newport, Shropshire, TF10 8NB, UK.
Theor Appl Genet. 2022 Jul;135(7):2481-2500. doi: 10.1007/s00122-022-04129-5. Epub 2022 Jun 8.
We demonstrate genetic variation for quantitative resistance against important fungal pathogens in lettuce and its wild relatives, map loci conferring resistance and predict key molecular mechanisms using transcriptome profiling. Lactuca sativa L. (lettuce) is an important leafy vegetable crop grown and consumed globally. Chemicals are routinely used to control major pathogens, including the causal agents of grey mould (Botrytis cinerea) and lettuce drop (Sclerotinia sclerotiorum). With increasing prevalence of pathogen resistance to fungicides and environmental concerns, there is an urgent need to identify sources of genetic resistance to B. cinerea and S. sclerotiorum in lettuce. We demonstrated genetic variation for quantitative resistance to B. cinerea and S. sclerotiorum in a set of 97 diverse lettuce and wild relative accessions, and between the parents of lettuce mapping populations. Transcriptome profiling across multiple lettuce accessions enabled us to identify genes with expression correlated with resistance, predicting the importance of post-transcriptional gene regulation in the lettuce defence response. We identified five genetic loci influencing quantitative resistance in a F mapping population derived from a Lactuca serriola (wild relative) × lettuce cross, which each explained 5-10% of the variation. Differential gene expression analysis between the parent lines, and integration of data on correlation of gene expression and resistance in the diversity set, highlighted potential causal genes underlying the quantitative trait loci.
我们在生菜及其野生近缘种中证明了对重要真菌病原体的定量抗性存在遗传变异，利用转录组谱分析定位了赋予抗性的基因座，并预测了关键的分子机制。生菜（Lactuca sativa L.）是一种重要的叶菜类蔬菜，在全球范围内种植和食用。为了控制包括灰霉病（Botrytis cinerea）和生菜凋萎病（Sclerotinia sclerotiorum）在内的主要病原体，人们通常会使用化学物质。随着病原菌对杀菌剂的抗药性日益普遍以及对环境的担忧加剧，迫切需要在生菜中鉴定出对 B. cinerea 和 S. sclerotiorum 的遗传抗性来源。我们在一组 97 种不同的生菜及其野生近缘种和生菜作图群体的亲本中证明了对 B. cinerea 和 S. sclerotiorum 的定量抗性存在遗传变异。在多个生菜品种上的转录组谱分析使我们能够识别出与抗性相关的表达基因，预测了在后转录基因调控在生菜防御反应中的重要性。我们在源自 Lactuca serriola（野生近缘种）×生菜杂交的 F 作图群体中鉴定出了五个影响定量抗性的遗传基因座，每个基因座解释了 5-10%的变异。在亲本系之间进行差异基因表达分析，并整合多样性群体中基因表达与抗性的相关性数据，突出了定量性状基因座的潜在因果基因。
