Ben-Amar Anis, Daldoul Samia, Reustle Götz M, Krczal Gabriele, Mliki Ahmed
Department of Plant Molecular Physiology, Center of Biotechnology of Borj Cedria, Tunisia;; AgroScience.GmbH, AlPlanta-Institute for Plant Research, Neustadt an der Weinstraße, Germany.
Department of Plant Molecular Physiology, Center of Biotechnology of Borj Cedria, Tunisia.
Curr Genomics. 2016 Dec;17(6):460-475. doi: 10.2174/1389202917666160520102827.
In the post-genomic era, increasingly sophisticated genetic tools are being developed with the long-term goal of understanding how the coordinated activity of genes gives rise to a complex organism. With the advent of the next generation sequencing associated with effective computational approaches, wide variety of plant species have been fully sequenced giving a wealth of data sequence information on structure and organization of plant genomes. Since thousands of gene sequences are already known, recently developed functional genomics approaches provide powerful tools to analyze plant gene functions through various gene manipulation technologies. Integration of different omics platforms along with gene annotation and computational analysis may elucidate a complete view in a system biology level. Extensive investigations on reverse genetics methodologies were deployed for assigning biological function to a specific gene or gene product. We provide here an updated overview of these high throughout strategies highlighting recent advances in the knowledge of functional genomics in plants.
在后基因组时代，人们正在开发越来越复杂的遗传工具，其长期目标是了解基因的协同活动如何产生一个复杂的生物体。随着与有效计算方法相关的下一代测序技术的出现，各种各样的植物物种已被完全测序，从而提供了关于植物基因组结构和组织的大量数据序列信息。由于已经知道了数千个基因序列，最近开发的功能基因组学方法提供了强大的工具，可通过各种基因操作技术来分析植物基因功能。整合不同的组学平台以及基因注释和计算分析，可能会在系统生物学层面阐明一个完整的图景。人们对反向遗传学方法进行了广泛研究，以赋予特定基因或基因产物生物学功能。我们在此提供这些高通量策略的最新概述，突出植物功能基因组学知识的最新进展。
