Qin Shidong, Zhu Bangqin, Huang Xingxing, Hull J Joe, Chen Lizhen, Luo Jing
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Insects. 2022 Aug 22;13(8):755. doi: 10.3390/insects13080755.
Jakovlev (Hemiptera: Miridae) is an omnivorous agricultural pest that has severe economic impacts on a diverse range of agricultural crops. Although the targeted disruption of reproductive development among insects has been proposed as a novel control strategy for pest species, the current understanding of the physiology and molecular mechanisms of reproduction is very limited. In this study, we isolated a putative () gene that is highly expressed in the fat body and ovaries of sexually mature females. The double-stranded RNA (dsRNA)-mediated knockdown of suppressed ovarian development and negatively impacted female fertility, which suggested that it plays an essential role in reproduction. The results of this study could help to expand our understanding of reproductive development and have the potential to facilitate the development of effective strategies for the better control of this pest species.
雅科夫列夫盲蝽（半翅目：盲蝽科）是一种杂食性农业害虫，对多种农作物造成严重经济影响。尽管针对昆虫生殖发育的定向干扰已被提议作为一种针对害虫物种的新型控制策略，但目前对生殖生理和分子机制的了解非常有限。在本研究中，我们分离出一个假定的（）基因，该基因在性成熟雌性的脂肪体和卵巢中高度表达。双链RNA（dsRNA）介导的该基因敲低抑制了卵巢发育，并对雌性生育力产生负面影响，这表明它在生殖中起重要作用。本研究结果有助于扩展我们对该物种生殖发育的理解，并有可能促进制定更有效控制该害虫物种的策略。
