Matilda Shiny C, Shanthi Chittibabu
School of Bioscience and Technology, VIT University, Vellore-632014, India.
J Basic Microbiol. 2017 May;57(5):402-412. doi: 10.1002/jobm.201600596. Epub 2017 Feb 20.
The changes induced in bacterial strains under stress conditions provide an insight into metal resistance strategies. Trivalent chromium resistant bacterium were isolated and identified by 16S rRNA gene sequencing and designated as Alcaligenes faecalis VITSIM2. The growth pattern was monitored. The organism also showed resistance to copper, cadmium, and certain antibiotics. The differentially expressed proteins in SDS PAGE were identified by mass spectrometry as flagellin and 50S ribosomal L36 protein. The morphological changes were identified by scanning electron microscopy. The changes in the cell wall content were estimated by peptidoglycan analysis and transformation of phosphates was detected by P NMR. Flow cytometry was employed to measure the membrane integrity, esterase activity and intracellular pH. In conclusion spectrum of proteomic, physiological, and morphological alterations was observed that aid the organism to overcome chromium stress.
在应激条件下细菌菌株所发生的变化有助于深入了解金属抗性策略。通过16S rRNA基因测序分离并鉴定了耐三价铬细菌，命名为粪产碱菌VITSIM2。监测了其生长模式。该菌株还表现出对铜、镉和某些抗生素的抗性。通过质谱鉴定SDS-PAGE中差异表达的蛋白质为鞭毛蛋白和50S核糖体L36蛋白。通过扫描电子显微镜鉴定形态变化。通过肽聚糖分析估计细胞壁含量的变化，并通过磷核磁共振检测磷酸盐的转化。采用流式细胞术测量膜完整性、酯酶活性和细胞内pH值。总之，观察到了蛋白质组学、生理学和形态学改变的谱，这些改变有助于该生物体克服铬胁迫。
