Zhang H H, Wang Y, Zhao C, Wang J, Zhang X L
Gansu Key Laboratory of Viticulture and Enology, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, Gansu, China.
Academy of State Administration of Grain, Beijing, China.
J Appl Microbiol. 2017 Sep;123(3):661-668. doi: 10.1111/jam.13537.
The aim of this study is to report on the hydrolytic action of Alcaligenes faecalis isolated from soil samples and its ability to degrade ochratoxin A.
An A. faecalis strain was identified and characterized by employing both a phenotypic analysis and 16S rDNA sequence analysis. The results show that this strain could degrade ochratoxin A efficiently but could not use it as a sole carbon source. Ochratoxin α was confirmed as a degradation product in the intracellular extract of A. faecalis using UPLC-MS/MS. Our results suggest that the biodegradation of ochratoxin A by the A. faecalis strain occurs through the hydrolysis of the ochratoxin A amide bond by a putative peptidase. This is the first report to date on the degradation of ochratoxin A by A. faecalis.
The A. faecalis strain is presumably a suitable candidate for use in the biodegradation of ochratoxin A.
Ochratoxin A, which is produced by some filamentous fungi, severely impacts human and animal health by contaminating several types of food and feed. Our study contributes to the identification of the function of A. faecalis 0D-1, which is capable of producing hydrolytic enzyme(s) to biodegrade ochratoxin A into nontoxic ochratoxin α, to minimize the risk associated with ochratoxin A exposure.
本研究旨在报告从土壤样本中分离出的粪产碱菌的水解作用及其降解赭曲霉毒素A的能力。
通过表型分析和16S rDNA序列分析对一株粪产碱菌进行了鉴定和表征。结果表明，该菌株能够高效降解赭曲霉毒素A，但不能将其作为唯一碳源。使用超高效液相色谱-串联质谱法（UPLC-MS/MS）在粪产碱菌的细胞内提取物中确认了赭曲霉毒素α为降解产物。我们的结果表明，粪产碱菌菌株对赭曲霉毒素A的生物降解是通过一种假定的肽酶水解赭曲霉毒素A的酰胺键来实现的。这是迄今为止关于粪产碱菌降解赭曲霉毒素A的首次报道。
粪产碱菌菌株可能是用于赭曲霉毒素A生物降解的合适候选菌株。
由一些丝状真菌产生的赭曲霉毒素A通过污染多种食品和饲料严重影响人类和动物健康。我们的研究有助于确定粪产碱菌0D-1的功能，该菌能够产生水解酶将赭曲霉毒素A生物降解为无毒的赭曲霉毒素α, 以将与接触赭曲霉毒素A相关的风险降至最低。
