Razmkhah Farnaz, Soleimani Masoud, Mehrabani Davood, Karimi Mohammad Hossein, Amini Kafi-Abad Sedigheh, Ramzi Mani, Iravani Saadi Mahdiyar, Kakoui Javad
1 Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
2 Stem cell technology research center, Shiraz University of Medical Sciences, Shiraz, Iran.
Tumour Biol. 2017 Feb;39(2):1010428317692234. doi: 10.1177/1010428317692234.
Microvesicles are released by different cell types and shuttle mRNAs and microRNAs which have the possibility to transfer genetic information to a target cell and alter its function. Acute myeloid leukemia is a malignant disorder, and leukemic cells occupy all the bone marrow microenvironment. In this study, we investigate the effect of leukemia microvesicles on healthy umbilical cord blood hematopoietic stem cells to find evidence of cell information transferring. Leukemia microvesicles were isolated from acute myeloid leukemia patients and were co-incubated with healthy hematopoietic stem cells. After 7 days, cell count, hematopoietic stem cell-specific cluster of differentiation (CD) markers, colony-forming unit assay, and some microRNA gene expressions were assessed. Data showed a higher number of hematopoietic stem cells after being treated with leukemia microvesicles compared with control (treated with no microvesicles) and normal (treated with normal microvesicles) groups. Also, increased levels of microRNA-21 and microRNA-29a genes were observed in this group, while colony-forming ability was still maintained and high ranges of CD34, CD34CD38, CD90, and CD117 phenotypes were observed as stemness signs. Our results suggest that leukemia microvesicles are able to induce some effects on healthy hematopoietic stem cells such as promoting cell survival and some microRNAs deregulation, while stemness is maintained.
微泡由不同细胞类型释放，并携带信使核糖核酸和微小核糖核酸，这些核酸有可能将遗传信息传递给靶细胞并改变其功能。急性髓系白血病是一种恶性疾病，白血病细胞占据了整个骨髓微环境。在本研究中，我们调查白血病微泡对健康脐带血造血干细胞的影响，以寻找细胞信息传递的证据。从急性髓系白血病患者中分离出白血病微泡，并将其与健康造血干细胞共同培养。7天后，评估细胞计数、造血干细胞特异性分化簇（CD）标志物、集落形成单位测定以及一些微小核糖核酸基因表达。数据显示，与对照组（未用微泡处理）和正常组（用正常微泡处理）相比，白血病微泡处理后的造血干细胞数量更多。此外，该组中微小核糖核酸-21和微小核糖核酸-29a基因水平升高，同时集落形成能力仍得以维持，并且观察到高水平的CD34、CD34CD38、CD90和CD117表型作为干性标志。我们的结果表明，白血病微泡能够对健康造血干细胞产生一些影响，如促进细胞存活和一些微小核糖核酸失调，同时维持干性。
