Winning Lewis, Robinson Leanne, Boyd Adrian R, El Karim Ikhlas A, Lundy Fionnuala T, Meenan Brian J
Centre for Experimental Medicine, The Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, 97 Lisburn Road, Belfast, Northern Ireland, BT9 7BL, United Kingdom.
Nanotechnology and Integrated Bioengineering Centre (NIBEC), School of Engineering, Ulster University, Shore Road, Newtownabbey, Co. Antrim, Northern Ireland, BT37 0QB, United Kingdom.
J Biomed Mater Res A. 2017 Jun;105(6):1692-1702. doi: 10.1002/jbm.a.36044. Epub 2017 Mar 27.
Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro-1 +ve periodontal ligament stem cells (PDLSCs) to three well-characterized biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nanotopography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP-TiTi). The CaP-TiTi surfaces were nonstoichiometric, carbonated, and calcium rich with a Ca/P ratio of 1.74. PDLSCs were grown on each surface in the absence of supplementary osteogneic-inducing agents. Osteoblastic responses were assessed for up to 21 days in culture by measuring gene expression using real time q-PCR and via assessment of intracellular alkaline phosphatase (ALP) activity. Gene expression analysis for the CaP-TiTi surfaces showed a significant late stage up-regulation of Secreted Phosphoprotein 1. Additionally, there was a significant up-regulation of the Wnt signaling genes β-catenin and Wnt Family Member 5 A on days 14 and 21, respectively for the CaP-TiTi surface. A significant increase in intracellular ALP at day 21 for the CaP-TiTi surface was also observed. These data suggest that the CaP-TiTi surfaces provide the bioactive conditions required for direct osteoblastic differentiation of PDLSCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1692-1702, 2017.
生物活性材料在牙种植学领域具有特殊的临床益处，在该领域中，干细胞向成骨细胞谱系的分化对于体内种植体的骨整合和正常功能是必需的。本研究的目的是评估Stro-1阳性牙周膜干细胞（PDLSCs）对三种特性明确的生物材料表面的成骨反应：磨损的钛表面（cpTi）作为对照；通过射频磁控溅射产生具有微观和纳米形貌的多晶钛表面（TiTi）；以及包含溅射沉积磷酸钙涂层的相同表面（CaP-TiTi）。CaP-TiTi表面是非化学计量的、含碳酸根且富含钙，钙磷比为1.74。PDLSCs在无补充成骨诱导剂的情况下在每个表面上生长。通过使用实时定量PCR测量基因表达并评估细胞内碱性磷酸酶（ALP）活性，在培养中长达21天评估成骨反应。CaP-TiTi表面的基因表达分析显示分泌型磷蛋白1在后期显著上调。此外，对于CaP-TiTi表面，Wnt信号基因β-连环蛋白和Wnt家族成员5A分别在第14天和第21天显著上调。在第21天还观察到CaP-TiTi表面的细胞内ALP显著增加。这些数据表明CaP-TiTi表面提供了PDLSCs直接成骨分化所需的生物活性条件。©2017威利期刊公司。《生物医学材料研究杂志》A部分：105A：1692 - 1702，2017年。
