Marconi Guya Diletta, Diomede Francesca, Pizzicannella Jacopo, Fonticoli Luigia, Merciaro Ilaria, Pierdomenico Sante D, Mazzon Emanuela, Piattelli Adriano, Trubiani Oriana
Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy.
ASL 02 Lanciano-Vasto-Chieti, SS. Annunziata Hospital, Chieti, Italy.
Front Cell Dev Biol. 2020 May 14;8:315. doi: 10.3389/fcell.2020.00315. eCollection 2020.
Bone formation, in skeletal development or in osseointegration processes, is the result of interaction between angiogenesis and osteogenesis. To establish osseointegration, cells must attach to the implant in a direct way without any deposition of soft tissue. Structural design and surface topography of dental implants enhance the cell attachment and can affect the biological response. The aim of the study was to evaluate the cytocompatibility, osteogenic and angiogenic markers involved in bone differentiation of human periodontal ligament stem cells (hPDLSCs) on different titanium disks surfaces. The hPDLSCs were cultured on pure titanium surfaces modified with two different procedures, sandblasted (Control-CTRL) and sandblasted/etched (Test-TEST) as experimental titanium surfaces. After 1 and 8 weeks of culture VEGF, VEGF-R, and RUNX2 expression was evaluated under confocal laser scanning microscopy. To confirm the obtained data, RT-PCR and WB analyses were performed in order to evaluate the best implant surface performance. TEST surfaces compared to CTRL titanium surfaces enhanced cell adhesion and increased VEGF and RUNX2 expression. Moreover, titanium TEST surfaces showed a different topographic morphology that promoted cell adhesion, proliferation, and osteogenic/angiogenic commitment. To conclude, TEST surfaces performed more efficiently than CTRL surfaces; furthermore, TEST surface results showed them to be more biocompatible, better tolerated, and appropriate for allowing hPDLSC growth and proliferation. This fact could also lead to more rapid bone-titanium integration.
在骨骼发育或骨结合过程中,骨形成是血管生成与骨生成相互作用的结果。为实现骨结合,细胞必须直接附着于植入物,而无软组织沉积。牙种植体的结构设计和表面形貌可增强细胞附着,并能影响生物学反应。本研究的目的是评估人牙周膜干细胞(hPDLSCs)在不同钛盘表面上参与骨分化的细胞相容性、成骨和血管生成标志物。将hPDLSCs培养在经过两种不同处理改性的纯钛表面上,即喷砂处理(对照-CTRL)和喷砂/蚀刻处理(测试-TEST)作为实验性钛表面。培养1周和8周后,在共聚焦激光扫描显微镜下评估VEGF、VEGF-R和RUNX2的表达。为证实所获数据,进行了RT-PCR和WB分析,以评估最佳的种植体表面性能。与CTRL钛表面相比,TEST表面增强了细胞黏附,并增加了VEGF和RUNX2的表达。此外,钛TEST表面呈现出不同的形貌形态,促进了细胞黏附、增殖以及成骨/血管生成趋向。总之,TEST表面的性能比CTRL表面更高效;此外,TEST表面的结果表明它们具有更高的生物相容性、更好的耐受性,并且适合hPDLSC的生长和增殖。这一事实也可能导致骨与钛的更快结合。