Daegu-Gyeongbuk Medical Innovation Foundation , Daegu 41061, Korea.
ACS Appl Mater Interfaces. 2017 Mar 15;9(10):8480-8489. doi: 10.1021/acsami.6b14800. Epub 2017 Mar 1.
The development of highly sensitive, stable, and biocompatible imaging agents allowing visualization of dendritic cell (DC) migration is one of the essential factors for effective DC-based immunotherapy. Here, we used a novel and efficient synthesis approach to develop radioiodine-124-labeled tannic acid gold core-shell nanoparticles (I-TA-Au@AuNPs) for DC labeling and in vivo tracking of their migration using positron emission tomography (PET). I-TA-Au@AuNPs were produced within 40 min in high yield via straightforward tannic acid-mediated radiolabeling chemistry and incorporation of Au shell, which resulted in high radio-sensitivity and excellent chemical stability of nanoparticles in DCs and living mice. I-TA-Au@AuNPs demonstrated good DC labeling efficiency and did not affect cell biological functions, including proliferation and phenotype marker expression. Importantly, I-TA-Au@AuNPs in an extremely low amount (0.1 mg/kg) were successfully applied to track the migration of DCs to lymphoid organs (draining lymph nodes) in mice.
开发高灵敏度、稳定且生物相容的成像剂，使其能够可视化树突状细胞 (DC) 的迁移，是有效基于 DC 的免疫疗法的关键因素之一。在这里，我们使用了一种新颖且高效的合成方法，开发了放射性碘 124 标记的单宁酸金核壳纳米粒子 (I-TA-Au@AuNPs)，用于 DC 标记和通过正电子发射断层扫描 (PET) 对其迁移进行体内追踪。通过简单的单宁酸介导的放射性标记化学和 Au 壳的掺入，在 40 分钟内以高产率制备了 I-TA-Au@AuNPs，这导致了纳米粒子在 DC 和活小鼠中的高放射灵敏度和优异的化学稳定性。I-TA-Au@AuNPs 表现出良好的 DC 标记效率，并且不影响细胞的生物学功能，包括增殖和表型标志物的表达。重要的是，以极低的量（0.1mg/kg）使用 I-TA-Au@AuNPs 成功地追踪了 DC 向小鼠淋巴结（引流淋巴结）的迁移。
