Rodrigues Amélia M G, Guimarães Denise O, Konno Tatiana U P, Tinoco Luzineide W, Barth Thiago, Aguiar Fernando A, Lopes Norberto P, Leal Ivana C R, Raimundo Juliana M, Muzitano Michelle F
Laboratório de Biologia do Reconhecer, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, 28013-602 Rio de Janeiro, Brazil.
Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro, Campus Macaé, Av. Aluízio da Silva Gomes, 50, Novo Cavaleiros, Macaé, 27930-560 Rio de Janeiro, Brazil.
Molecules. 2017 Feb 18;22(2):304. doi: 10.3390/molecules22020304.
The aim of this research was to perform a phytochemical study of the methanol leaves extract of (MET) guided by vasodilatory and antioxidant activities. The chemical profile of MET and the ethyl acetate fraction (EA fraction) was determined by HPLC-UV-MS and EA fraction guided fractionation by reverse-phase chromatography. The vasorelaxant effects of MET, fractions, sub-fractions and constituents were assessed on rat aorta pre-contracted with phenylephrine. Antioxidant activity was evaluated by using a DPPH assay. The results show that MET-induced vasodilation was dependent on NO/cGMP; and that the PI3K/Akt pathway seems to be the main route involved in eNOS activation. The EA fraction showed greater vasodilatory and antioxidant potency and was submitted to further fractionation. This allowed the isolation and characterization of quercetin, quercetin 3--(6″--galloyl)-β-d-galactopyranoside and 1,4,6-tri--galloyl-β-d-glucose. Also, galloyl-HHDP-hexoside and myricetin deoxyhexoside were identified by HPLC-UV-MS. These compounds are being described for the first time for . 1,4,6-tri--galloyl-β-d-glucose and quercetin 3--(6″--galloyl)-β-d-galactopyranoside showed no vasodilatory activity. Quercetin and myricetin glycoside seems to contribute to the MET activity, since they have been reported as vasodilatory flavonoids. MET-induced vasodilation could contribute to the hypotensive effect of previously reported.
本研究的目的是在血管舒张和抗氧化活性的指导下，对[植物名称]甲醇叶提取物（MET）进行植物化学研究。通过高效液相色谱-紫外-质谱联用（HPLC-UV-MS）测定MET和乙酸乙酯馏分（EA馏分）的化学图谱，并通过反相色谱对EA馏分进行导向分级分离。在苯肾上腺素预收缩的大鼠主动脉上评估MET、馏分、亚馏分和成分的血管舒张作用。通过二苯基苦味酰基自由基（DPPH）测定法评估抗氧化活性。结果表明，MET诱导的血管舒张依赖于一氧化氮/环磷酸鸟苷（NO/cGMP）；并且磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/Akt）途径似乎是参与内皮型一氧化氮合酶（eNOS）激活的主要途径。EA馏分显示出更大的血管舒张和抗氧化效力，并进一步进行了分级分离。这使得槲皮素、槲皮素3 - O -（6″ - O - 没食子酰基）-β - D - 吡喃半乳糖苷和1,4,6 - 三 - O - 没食子酰基-β - D - 葡萄糖得以分离和鉴定。此外，通过HPLC-UV-MS鉴定出了没食子酰基-HHDP-己糖苷和杨梅素脱氧己糖苷。这些化合物首次在[植物名称]中被描述。1,4,6 - 三 - O - 没食子酰基-β - D - 葡萄糖和槲皮素3 - O -（6″ - O - 没食子酰基）-β - D - 吡喃半乳糖苷未显示出血管舒张活性。槲皮素和杨梅素糖苷似乎对MET的活性有贡献，因为它们已被报道为血管舒张类黄酮。MET诱导的血管舒张可能有助于先前报道的[植物名称]的降压作用。
