Ren Guixing, Zhu Yingying, Shi Zhenxing, Li Jianhui
College of Pharmacy and Biological Engineering, Chengdu University, Chengdu, China.
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
J Sci Food Agric. 2017 Sep;97(12):4110-4116. doi: 10.1002/jsfa.8278. Epub 2017 Apr 6.
Lunasin is a novel cancer-preventive peptide that has been detected in various plants. However, the presence and bioactivity of lunasin in quinoa have not been demonstrated.
Lunasin was detected in quinoa using ultrahigh-pressure liquid chromatography-electrosparay ionization-mass spectrometry. The content in 15 quinoa samples ranged from 1.01 × 10 g kg dry seed to 4.89 × 10 g kg dry seed. Significant differences (P < 0.05) in lunasin content among different cultivars (yy22, xsg86, xsg59, yy30 and yy19) from the same area and the same cultivar from different areas (Hainan, Hebei and Shanxi provinces) were observed. The lunasin isolated and purified from quinoa (QLP) exhibited a weak DPPH radical scavenging activity (no IC value), but a strong ABTS radical scavenging activity (IC value, 1.45 g L ) and oxygen radical scavenging activity (40.06 µmol L Trolox equivalents/g QLP when the concentration was 3.20 g L ). In addition, QLP inhibited the production of nitric oxide (NO), tumor necrosis factor-α and interleukin-6 on lipopolysaccharide-stimulated RAW264.7 macrophages by up to 44.77%, 39.81% and 33.50%, respectively, at a concentration of 0.40 g L .
Taken together, these findings indicate that lunasin presents in quinoa and is bioactive, which strengthens the recommendations for the development of quinoa-based functional foods. © 2017 Society of Chemical Industry.
芦那辛是一种在多种植物中被检测到的新型防癌肽。然而，藜麦中芦那辛的存在及其生物活性尚未得到证实。
采用超高压液相色谱 - 电喷雾电离 - 质谱法在藜麦中检测到了芦那辛。15个藜麦样品中的含量范围为1.01×10克/千克干种子至4.89×10克/千克干种子。观察到来自同一地区的不同品种（yy22、xsg86、xsg59、yy30和yy19）以及来自不同地区（海南、河北和山西省）的同一品种之间芦那辛含量存在显著差异（P < 0.05）。从藜麦中分离纯化得到的芦那辛（QLP）表现出较弱的DPPH自由基清除活性（无IC值），但具有较强的ABTS自由基清除活性（IC值为1.45克/升）和氧自由基清除活性（当浓度为3.20克/升时，为40.06微摩尔/升Trolox当量/克QLP）。此外，在浓度为0.40克/升时，QLP对脂多糖刺激的RAW264.7巨噬细胞中一氧化氮（NO）、肿瘤坏死因子 -α和白细胞介素 -6的产生分别抑制高达44.77%、39.81%和33.50%。
综上所述，这些发现表明藜麦中存在芦那辛且具有生物活性，这加强了开发基于藜麦的功能性食品的建议。© 2017化学工业协会。
