Hong Sang Pil, Shin Seung Chul, Kang Woo-Kyung, Chin Young-Wook, Turner Timothy L, Choi Hyun Wook, Song Kyung Mo, Kim Hyo Jin
Division of Platform Technology Research, Korea Food Research Institute, Seongnam 13539, Republic of Korea.
Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of Korea.
J Biotechnol. 2017 Mar 20;246:40-44. doi: 10.1016/j.jbiotec.2017.02.018. Epub 2017 Feb 17.
Leuconostoc garlicum KCCM 43211 isolated from traditional Korean fermented food is an intensive producer of exopolysaccharide (EPS). Here we report the first complete genome sequence of L. garlicum KCCM 43211. The genome sequence displayed that this strain contains genes involved in production of EPS possibly composed of glucose monomers. An uncharacterized EPS from the L. garlicum KCCM 43211 strains was also produced during fermentation in the sucrose medium. The MALDI-TOF results displayed the typical mass spectrometry pattern of dextran. This uncharacterized EPS may have use in commercial prebiotics, food additives, and medical purposes. The complete genome sequence of L. garlicum KCCM 43211 will provide valuable information for strain engineering based on the genetic information.
从传统韩国发酵食品中分离出的大蒜明串珠菌KCCM 43211是一种胞外多糖(EPS)高产菌株。在此,我们报道了大蒜明串珠菌KCCM 43211的首个完整基因组序列。基因组序列显示,该菌株含有参与可能由葡萄糖单体组成的EPS生产的基因。在蔗糖培养基发酵过程中,大蒜明串珠菌KCCM 43211菌株还产生了一种未鉴定的EPS。基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)结果显示了典型的葡聚糖质谱图。这种未鉴定的EPS可能用于商业益生元、食品添加剂和医疗用途。大蒜明串珠菌KCCM 43211的完整基因组序列将基于遗传信息为菌株工程提供有价值的信息。