Li Wenting, Wang Ying, Fang Xinzhi, Zhou Mei, Li Yiqun, Dong Ying, Wang Ruozheng
Department of Pathology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China (mainland).
Department of Medical Administration, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China (mainland).
Med Sci Monit. 2017 Feb 21;23:938-947. doi: 10.12659/msm.902267.
BACKGROUND The aim of this study was to investigate the expression and the clinicopathologic significance of DNA methyltransferase 3B (DNMT3B), phosphatase and tensin homolog (PTEN) and human MutL homologs 1 (hMLH1) in endometrial carcinomas between Han and Uygur women in Xinjiang. MATERIAL AND METHODS The expression of DNMT3B, PTEN, and hMLH1 in endometrial carcinomas were assessed by immunohistochemistry, followed by an analysis of their relationship to clinical-pathological features and prognosis. RESULTS There were a 61.7% (95/154) overexpression of DNMT3B, 50.0% (77/154) loss of PTEN expression and 18.2% (28/154) loss of hMLH1 expression. The expression of DNMT3B and PTEN in endometrial carcinomas was statistically significantly different between Uygur women and Han women (p=0.001, p=0.010, respectively). DNMT3B expression was statistically significant based on the grade of endometrial carcinomas (p=0.031). PTEN loss was statistically significant between endometrioid carcinomas (ECs) and non endometrioid carcinomas (NECs) (p=0.040). DNMT3B expression was statistically significant in different myometrial invasion groups in Uygur women (p=0.010). Furthermore, the correlation of DNMT3B and PTEN expression was significant in endometrial carcinomas (p=0.021). PTEN expression was statistically significant in the overall survival (OS) rate of women with endometrial cancers (p=0.041). CONCLUSIONS Our findings suggest that PTEN and DNMT3B possess common regulation features as well as certain ethnic differences in expression between Han women and Uygur women. An interaction may exist in the pathogenesis of endometrial carcinoma. DNMT3B was expressed differently in cases of myometrial invasion and PTEN was associated with OS, which suggested that these molecular markers may be useful in the evaluation of the biological behavior of endometrial carcinomas and may be useful indicators of prognosis in women with endometrial carcinomas.
背景 本研究旨在探讨DNA甲基转移酶3B（DNMT3B）、磷酸酶和张力蛋白同源物（PTEN）以及人MutL同源物1（hMLH1）在新疆汉族和维吾尔族女性子宫内膜癌中的表达及其临床病理意义。材料与方法 采用免疫组织化学法评估DNMT3B、PTEN和hMLH1在子宫内膜癌中的表达，随后分析它们与临床病理特征及预后的关系。结果 DNMT3B过表达率为61.7%（95/154），PTEN表达缺失率为50.0%（77/154），hMLH1表达缺失率为18.2%（28/154）。维吾尔族女性和汉族女性子宫内膜癌中DNMT3B和PTEN的表达在统计学上有显著差异（分别为p = 0.001，p = 0.010）。DNMT3B的表达根据子宫内膜癌的分级在统计学上有显著差异（p = 0.031）。PTEN缺失在子宫内膜样癌（ECs）和非子宫内膜样癌（NECs）之间在统计学上有显著差异（p = 0.040）。DNMT3B的表达在维吾尔族女性不同肌层浸润组中在统计学上有显著差异（p = 0.010）。此外，DNMT3B和PTEN的表达在子宫内膜癌中具有显著相关性（p = 0.021）。PTEN的表达在子宫内膜癌女性的总生存率（OS）方面在统计学上有显著差异（p = 0.041）。结论 我们的研究结果表明，PTEN和DNMT3B具有共同的调控特征，并且在汉族女性和维吾尔族女性之间的表达存在一定的种族差异。子宫内膜癌的发病机制中可能存在相互作用。DNMT3B在肌层浸润病例中的表达不同，PTEN与OS相关，这表明这些分子标志物可能有助于评估子宫内膜癌的生物学行为，并且可能是子宫内膜癌女性预后的有用指标。
