肾二肽酶是磷脂酰肌醇特异性磷脂酶C释放的膜蛋白之一。
Renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase C.
作者信息
Hooper N M, Low M G, Turner A J
机构信息
Department of Biochemistry, University of Leeds, U.K.
出版信息
Biochem J. 1987 Jun 1;244(2):465-9. doi: 10.1042/bj2440465.
Abstract
Renal dipeptidase (dehydropeptidase-I, EC 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase C from Staphylococcus aureus and Bacillus thuringiensis and a phospholipase C preparation from Bacillus cereus to a similar extent as alkaline phosphatase. Endopeptidase-24.11 and aminopeptidase N were not released by this treatment. After treatment of the membrane fraction with the S. aureus phospholipase C the dipeptidase was converted from an amphipathic to a hydrophilic form, as deduced from phase-separation experiments in Triton X-114. It is concluded that renal dipeptidase is anchored to the microvillar membrane by covalently attached phosphatidylinositol.
摘要
用来自金黄色葡萄球菌、苏云金芽孢杆菌的磷脂酰肌醇特异性磷脂酶C以及蜡状芽孢杆菌的磷脂酶C制剂处理猪肾膜制剂，肾二肽酶（脱氢肽酶-I，EC 3.4.13.11）的释放程度与碱性磷酸酶相似。这种处理不会释放内肽酶-24.11和氨肽酶N。用金黄色葡萄球菌磷脂酶C处理膜部分后，根据Triton X-114中的相分离实验推断，二肽酶从两亲形式转变为亲水形式。得出的结论是，肾二肽酶通过共价连接的磷脂酰肌醇锚定在微绒毛膜上。
相似文献
1
Renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase C.肾二肽酶是磷脂酰肌醇特异性磷脂酶C释放的膜蛋白之一。
Biochem J. 1987 Jun 1;244(2):465-9. doi: 10.1042/bj2440465.
2
Activation of the glycosyl-phosphatidylinositol-anchored membrane dipeptidase upon release from pig kidney membranes by phospholipase C.通过磷脂酶C从猪肾膜释放后糖基磷脂酰肌醇锚定膜二肽酶的激活。
Biochem J. 1994 Oct 15;303 ( Pt 2)(Pt 2):633-8. doi: 10.1042/bj3030633.
3
cDNA cloning and expression in Xenopus laevis oocytes of pig renal dipeptidase, a glycosyl-phosphatidylinositol-anchored ectoenzyme.猪肾二肽酶（一种糖基磷脂酰肌醇锚定的胞外酶）的cDNA克隆及其在非洲爪蟾卵母细胞中的表达
Biochem J. 1990 Nov 1;271(3):755-60. doi: 10.1042/bj2710755.
4
Identification of membrane dipeptidase as a major glycosyl-phosphatidylinositol-anchored protein of the pancreatic zymogen granule membrane, and evidence for its release by phospholipase A.鉴定膜二肽酶为胰腺酶原颗粒膜的一种主要糖基磷脂酰肌醇锚定蛋白，并证明其可被磷脂酶A释放。
Biochem J. 1997 May 15;324 ( Pt 1)(Pt 1):151-7. doi: 10.1042/bj3240151.
5
Solubilization of trehalase from rabbit renal and intestinal brush-border membranes by a phosphatidylinositol-specific phospholipase C.磷脂酰肌醇特异性磷脂酶C对兔肾和肠刷状缘膜中海藻糖酶的增溶作用。
FEBS Lett. 1986 May 26;201(1):5-8. doi: 10.1016/0014-5793(86)80560-9.
6
Ectoenzymes of the kidney microvillar membrane. Differential solubilization by detergents can predict a glycosyl-phosphatidylinositol membrane anchor.肾微绒毛膜的外切酶。去污剂的差异增溶作用可预测糖基磷脂酰肌醇膜锚定物。
Biochem J. 1988 Mar 15;250(3):865-9. doi: 10.1042/bj2500865.
7
Bioconversion of leukotriene D4 by lung dipeptidase.
Biochim Biophys Acta. 1990 Jan 16;1042(1):107-12. doi: 10.1016/0005-2760(90)90063-4.
8
Ectoenzymes of the kidney microvillar membrane. Affinity purification, characterization and localization of the phospholipase C-solubilized form of renal dipeptidase.肾微绒毛膜的外切酶。肾二肽酶磷脂酶C可溶形式的亲和纯化、特性鉴定及定位
Biochem J. 1989 Jan 15;257(2):361-7. doi: 10.1042/bj2570361.
9
Tetrameric alkaline phosphatase from human liver is converted to dimers by phosphatidylinositol phospholipase C.来自人肝脏的四聚体碱性磷酸酶被磷脂酰肌醇磷脂酶C转化为二聚体。
FEBS Lett. 1987 Feb 23;212(2):289-91. doi: 10.1016/0014-5793(87)81362-5.
10
引用本文的文献
1
Cilastatin Modulates DPEP1- and IQGAP1-Associated Neuro-Glio-Vascular Inflammation in Oxaliplatin-Induced Peripheral Neurotoxicity.西司他丁调节奥沙利铂诱导的周围神经毒性中与二肽基肽酶1（DPEP1）和IQGAP1相关的神经-胶质-血管炎症。
Cells. 2025 Aug 20;14(16):1294. doi: 10.3390/cells14161294.
2
DPEP1 Balance GSH Involve in Cadmium Stress Response in Blood Clam .DPEP1平衡谷胱甘肽参与血蚶对镉胁迫的响应 。
Front Physiol. 2018 Jul 20;9:964. doi: 10.3389/fphys.2018.00964. eCollection 2018.
3
Dehydropeptidase 1 promotes metastasis through regulation of E-cadherin expression in colon cancer.脱氢肽酶1通过调控结肠癌中E-钙黏蛋白的表达促进转移。
Oncotarget. 2016 Feb 23;7(8):9501-12. doi: 10.18632/oncotarget.7033.
4
D-Aspartyl residue in a peptide can be liberated and metabolized by pig kidney enzymes.肽中的 D-天冬氨酸残基可以被猪肾酶释放和代谢。
Amino Acids. 1996 Jun;10(2):187-96. doi: 10.1007/BF00806591.
5
Rostral growth of commissural axons requires the cell adhesion molecule MDGA2.连合轴突的前向生长需要细胞黏附分子 MDGA2。
Neural Dev. 2011 May 4;6:22. doi: 10.1186/1749-8104-6-22.
6
DPEP1, expressed in the early stages of colon carcinogenesis, affects cancer cell invasiveness.DPEP1 在结直肠癌发生的早期表达，影响癌细胞的侵袭性。
J Gastroenterol. 2011 Feb;46(2):153-63. doi: 10.1007/s00535-010-0318-1. Epub 2010 Sep 8.
7
Differential effects of glycosphingolipids on the detergent-insolubility of the glycosylphosphatidylinositol-anchored membrane dipeptidase.糖鞘脂对糖基磷脂酰肌醇锚定膜二肽酶去污剂不溶性的差异影响。
Biochem J. 2001 Aug 15;358(Pt 1):209-16. doi: 10.1042/0264-6021:3580209.
8
Roles of the juxtamembrane and extracellular domains of angiotensin-converting enzyme in ectodomain shedding.血管紧张素转换酶的近膜区和细胞外结构域在胞外域脱落中的作用。
Biochem J. 2001 Aug 15;358(Pt 1):185-92. doi: 10.1042/0264-6021:3580185.
9
Amyloid precursor protein, although partially detergent-insoluble in mouse cerebral cortex, behaves as an atypical lipid raft protein.淀粉样前体蛋白，尽管在小鼠大脑皮层中部分不溶于去污剂，但表现为一种非典型的脂筏蛋白。
Biochem J. 1999 Nov 15;344 Pt 1(Pt 1):23-30.
10
Identification by site-directed mutagenesis of three essential histidine residues in membrane dipeptidase, a novel mammalian zinc peptidase.通过定点诱变鉴定膜二肽酶（一种新型哺乳动物锌肽酶）中的三个必需组氨酸残基。
Biochem J. 1997 Aug 15;326 ( Pt 1)(Pt 1):47-51. doi: 10.1042/bj3260047.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Purification and properties of phosphatidylinositol-specific phospholipase C of Bacillus thuringiensis.苏云金芽孢杆菌磷脂酰肌醇特异性磷脂酶C的纯化及性质
Biochim Biophys Acta. 1980 Jul 14;619(1):48-57.
3
Metabolism of thienamycin and related carbapenem antibiotics by the renal dipeptidase, dehydropeptidase.硫霉素及相关碳青霉烯类抗生素在肾脏二肽酶即脱氢肽酶作用下的代谢
Antimicrob Agents Chemother. 1982 Jul;22(1):62-70. doi: 10.1128/AAC.22.1.62.
4
Association of renal dipeptidase with the Triton-insoluble fraction of kidney microvilli.肾二肽酶与肾微绒毛的不溶性 Triton 组分的关联。
J Membr Biol. 1983;75(2):115-22. doi: 10.1007/BF01995631.
5
Thienamycin: development of imipenen-cilastatin.硫霉素：亚胺培南-西司他丁的研发
J Antimicrob Chemother. 1983 Dec;12 Suppl D:1-35. doi: 10.1093/jac/12.suppl_d.1.
6
Phase separation of integral membrane proteins in Triton X-114 solution.整合膜蛋白在Triton X-114溶液中的相分离。
J Biol Chem. 1981 Feb 25;256(4):1604-7.
7
Topology of microvillar membrance hydrolases of kidney and intestine.肾脏和肠道微绒毛膜水解酶的拓扑结构
Physiol Rev. 1982 Jan;62(1):91-128. doi: 10.1152/physrev.1982.62.1.91.
8
Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein.糖基化的sn-1,2-二肉豆蔻酰磷脂酰肌醇与布氏锥虫可变表面糖蛋白共价连接。
J Biol Chem. 1985 Nov 25;260(27):14547-55.
9
Solubilization of membrane-bound acetylcholinesterase by a phosphatidylinositol-specific phospholipase C.磷脂酰肌醇特异性磷脂酶C对膜结合型乙酰胆碱酯酶的增溶作用。
J Neurochem. 1985 Nov;45(5):1487-94. doi: 10.1111/j.1471-4159.1985.tb07217.x.
10
Phosphatidylinositol is involved in the membrane attachment of NCAM-120, the smallest component of the neural cell adhesion molecule.磷脂酰肌醇参与神经细胞黏附分子最小成分NCAM - 120的膜附着。
EMBO J. 1986 Oct;5(10):2489-94. doi: 10.1002/j.1460-2075.1986.tb04526.x.
Zotero 插件