Skolkovo Institute of Science and Technology, 3 Nobel Street, Innovation Center "Skolkovo", 143026 Skolkovo, Russia.
Research Institute of Occupational Health, Prospekt Budenogo 31, 105275 Moscow, Russia.
Analyst. 2017 Mar 13;142(6):945-950. doi: 10.1039/c6an02441f.
Molecular beacons (MBs) are valuable tools in molecular biology, clinical diagnostics and analytical chemistry. Here we describe a novel approach for the design of MBs with nucleotide or non-nucleotide linkers between the stem and loop regions. Such modified MBs have significantly improved specificity and performance for single nucleotide polymorphism (SNP) detection. These advantages are especially distinct, when compared to the classic MBs, in the case of possible interactions between the stem and loop regions. We demonstrated the applicability of such modified MBs for the discrimination of common Factor V, NOS3 and ADRB2 SNPs in model plasmids and in clinical samples. The developed approach could be applicable not only to fluorescently labeled MBs, but also to other biosensors based on nucleic acids with stem-loop structures.
分子信标 (MBs) 是分子生物学、临床诊断和分析化学中非常有价值的工具。在这里，我们描述了一种在茎环区域之间使用核苷酸或非核苷酸接头设计 MBs 的新方法。这种修饰的 MBs 显著提高了用于单核苷酸多态性 (SNP) 检测的特异性和性能。与经典的 MBs 相比，当茎环区域之间可能存在相互作用时，这些优势尤为明显。我们在模型质粒和临床样本中证明了这种修饰的 MBs 用于区分常见的因子 V、NOS3 和 ADRB2 SNPs 的适用性。所开发的方法不仅可以应用于荧光标记的 MBs，还可以应用于基于具有茎环结构的核酸的其他生物传感器。
