用于常规血液学检测的二钾和三钾乙二胺四乙酸（EDTA）真空采血管的评估。

Evaluation of di-potassium and tri-potassium EDTA evacuated tubes for routine haematological testing.

作者信息

Mehmood Rubaida, Muhammed Rabia Khushi, Hussain Sajjid, Sana Aniqa

机构信息

PAEC, MINAR, Multan, Pakistan.

Department of Chemistry, Women University Multan, Multan, Pakistan.

出版信息

J Clin Lab Anal. 2018 Jan;32(1). doi: 10.1002/jcla.22188. Epub 2017 Feb 21.

DOI:10.1002/jcla.22188

PMID:28220977

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6817102/

Abstract

BACKGROUND

The influence of blood collection devices on laboratory tests is often overlooked. We have discussed here how blood collection materials and devices can alter haematological test results, with an emphasis on blood collection tube (BCT) additives. We have compared K2 & K3-EDTA vacuum tubes of same manufacturer IMPROVACUTER for CBC analysis.

METHODS

Blood was collected in both tubes simultaneously. Samples were analysed immediately after collection (<15 minutes) and after 4 hours by MEK-6318J/K Haematology Analyzer. Paired student's t-test was applied for statistical analysis. Significant differences among results and biases were compared with current quality specifications.

RESULTS

Significant differences were found in Neutrophyls (GR) and mean platelets volume (MPV) when compared. K2-EDTA vs K3-EDTA(1st time) after< 15 minutes of sample collection, for Monocytes counts (MO), MCV, MPV, and RDW when comparing K2-EDTA (first time) vs K2-EDTA(second time, after 4 hours); and for Monocytes counts (MO), MCV, MPV, PCT, and RDW when comparing K3-EDTA(1st time) vs K3-EDTA(2nd time) in order to check the stability and reproducibility of vacutainers.

CONCLUSIONS

This study assesses the use of K2& K3-EDTA vacuum tubes whether or not they represent a bias in haematological testing (CBC). The use of K2-EDTA and K3-EDTA vacuum tubes represent a clinically relevant source of variation for MCV, MPV, RDW, and PCT.

摘要

背景

采血设备对实验室检测的影响常常被忽视。我们在此讨论了采血材料和设备如何改变血液学检测结果，重点是采血管（BCT）添加剂。我们比较了同一制造商IMPROVACUTER生产的用于全血细胞计数分析的K2和K3乙二胺四乙酸（EDTA）真空管。

方法

同时在两根真空管中采血。采集后立即（<15分钟）和4小时后，使用MEK - 6318J/K血液分析仪对样本进行分析。采用配对学生t检验进行统计分析。将结果和偏差的显著差异与当前质量规范进行比较。

结果

比较时发现中性粒细胞（GR）和平均血小板体积（MPV）存在显著差异。样本采集<15分钟后，比较K2 - EDTA与K3 - EDTA（首次）时，单核细胞计数（MO）、平均红细胞体积（MCV）、MPV和红细胞分布宽度（RDW）存在差异；比较K2 - EDTA（首次）与K2 - EDTA（第二次，4小时后）时，单核细胞计数（MO）、MCV、MPV、血小板压积（PCT）和RDW存在差异；比较K3 - EDTA（首次）与K3 - EDTA（第二次）时，单核细胞计数（MO）、MCV、MPV、PCT和RDW存在差异，以检查真空采血管的稳定性和重复性。