不同类型采血管中完整甲状旁腺激素（PTH）的稳定性。

The Stability of Intact Parathyroid Hormone (PTH) in Different Types of Blood Collection Tubes.

出版信息

Clin Lab. 2022 Mar 1;68(3). doi: 10.7754/Clin.Lab.2021.210421.

DOI:10.7754/Clin.Lab.2021.210421

Abstract

BACKGROUND

Over past decades, the instability of parathyroid hormone (PTH) causes great interference for the clinical laboratory. Contradictory results were reported in many reports about storage conditions and suitable blood collection tubes to ensure PTH stability in the pretreatment phase.

METHODS

This study recruited 30 participants including 10 healthy persons, 10 hemodialysis, and 10 hyperparathyroidism patients. Five types of blood collection tubes (EDTA-K3 tube, coagulant tube, heparin anticoagulant tube, gel separating tube, and plain tube) were included to determine whether they were suitable as blood-collecting vessels. The time points and conditions for testing samples included less than 2 hours, 4 hours, and 8 hours at room temperature, and, in parallel, 24 hours, 48 hours, and 72 hours in refrigeration. Two different judgement criteria were used to compare the stability of PTH in different blood vessels.

RESULTS

Purely statistical analysis showed that 4 types of blood collection tubes could not perform the same storage ability as EDTA-K3 tube at "T0" time point. Plain tube had the largest drop among all types of blood collection tubes. Compared by pairwise t-test, EDTA-K3 tube could maintain intact PTH for 8 hours (p = 0.998) at room temperature and 24 hours (p = 0.053) in refrigeration. When comparing the total change limit (TCL = 18.8%), at room temperature, EDTA-K3 tube (7.0%), heparin tube (12.7%), coagulant tube (16.2%), and plain tube (17.6%) could maintain intact PTH for 8 hours, and GST can preserve PTH for 4 hours (18.2%). In refrigeration, EDTA-K3 tube could maintain PTH for 72 hours (7.5%) and heparin tube could maintain 24 hours (18.4%). The other three blood collection tubes could not preserve PTH in refrigeration (GST = 22.1%, coagulant tube = 20.3%, plain tube = 20.8%).

CONCLUSIONS

PTH seems more stable in the EDTA-K3 tube than any other blood collection tubes and is followed next by the heparin anticoagulant tube. Plain tube and GST have faster degradation than other tubes and are not suggested to preserve intact PTH.

摘要

背景

过去几十年，甲状旁腺激素（PTH）的不稳定性对临床实验室造成了很大的干扰。关于保证预处理阶段 PTH 稳定性的储存条件和合适的采血试管，许多报告中都有相互矛盾的结果。

方法

本研究纳入了 30 名参与者，包括 10 名健康人、10 名血液透析患者和 10 名甲状旁腺功能亢进症患者。共纳入 5 种采血管（EDTA-K3 管、凝血管、肝素抗凝管、凝胶分离管和普通管），以确定它们是否适合作为采血容器。检测样本的时间点和条件包括室温下 2 小时、4 小时和 8 小时，以及冷藏 24 小时、48 小时和 72 小时。使用两种不同的判断标准来比较不同采血管中 PTH 的稳定性。

结果

单纯的统计学分析表明，在“T0”时间点，4 种采血管均不能像 EDTA-K3 管一样具有相同的储存能力。普通管在所有采血管中下降最大。通过配对 t 检验比较，EDTA-K3 管在室温下可保持完整的 PTH 8 小时（p = 0.998），在冷藏条件下可保持完整的 PTH 24 小时（p = 0.053）。当比较总变化限制（TCL = 18.8%）时，在室温下，EDTA-K3 管（7.0%）、肝素管（12.7%）、凝血管（16.2%）和普通管（17.6%）可保持完整的 PTH 8 小时，GST 可保持 4 小时（18.2%）。在冷藏条件下，EDTA-K3 管可保持 PTH 72 小时（7.5%），肝素管可保持 24 小时（18.4%）。其他三种采血管在冷藏条件下均不能保存 PTH（GST = 22.1%、凝血管 = 20.3%、普通管 = 20.8%）。